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SLUG Antibody (YA3542)

(Synonyms: SLUG; WS2D; SLUGH1; SNAIL2)
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Based on 1 Customer Validation

SLUG Antibody (YA3542) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLUG.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SLUG Antibody (YA3542)
  • Experimental Validation Results for SLUG Antibody (YA3542)
  • SLUG Antibody (YA3542) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

SLUG Antibody (YA3542) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLUG.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 30 kDa
Immunogen

Purified recombinant fragment of human SNAI2 (AA: 100-200) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718780

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SLUG Antibody (YA3542)
    Western blot analysis of extracts from U87 (lane 2(20μg), 3T3-L1 (lane 3(20μg), A549 (lane 4(20μg), A431 (lane 5(20μg) using SLUG Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SLUG Antibody (YA3542)
    Flow Cytometry analysis of MCF-7 cells labelling SLUG (red) with SLUG Antibody (anti-SLUG) (HY-P83845). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P807578, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    SLUG transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:It is expressed in most adult tissues, including the spleen, thymus, prostate, testes, ovaries, small intestine, colon, heart, brain, placenta, lungs, liver, skeletal muscle, kidneys, and pancreas. It is not detected in peripheral blood leukocytes. It is expressed in all layers of the dermis and epidermis, with higher expression levels in the basal layer (protein level) . It is expressed in osteoblasts (protein level) . It is expressed in mesenchymal stem cells (protein level) . It is expressed in breast tumor cells (protein level) .

  • Subunit

    Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains) (By similarity). Interacts (via zinc fingers) with KPNA2, KPNB1, and TNPO1. May interact (via zinc fingers) with IPO7

  • SwissProt ID

    O43623

  • Gene ID
    6591 [NCBI]
  • Synonyms

    SLUG; WS2D; SLUGH1; SNAIL2

SLUG Antibody (YA3542) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U87 (lane 2(20μg), 3T3-L1 (lane 3(20μg), A549 (lane 4(20μg), A431 (lane 5(20μg) using SLUG Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow Cytometry analysis of MCF-7 cells labelling SLUG (red) with SLUG Antibody (anti-SLUG) (HY-P83845). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P807578, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SLUG Antibody (YA3542) Product Image
    1/16
  • Experimental Validation Results for SLUG Antibody (YA3542)
  • Experimental Validation Results for SLUG Antibody (YA3542)
  • SLUG Antibody (YA3542) Product Image
  • Experimental Validation Results for SLUG Antibody (YA3542)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U87 (lane 2(20μg), 3T3-L1 (lane 3(20μg), A549 (lane 4(20μg), A431 (lane 5(20μg) using SLUG Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SLUG Antibody (YA3542)
    1/1
    MedChemExpress Validation
     Flow Cytometry analysis of MCF-7 cells labelling SLUG (red) with SLUG Antibody (anti-SLUG) (HY-P83845). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P807578, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for SLUG Antibody (YA3542)
  • Experimental Validation Results for SLUG Antibody (YA3542)
  • SLUG Antibody (YA3542) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U87 (lane 2(20μg), 3T3-L1 (lane 3(20μg), A549 (lane 4(20μg), A431 (lane 5(20μg) using SLUG Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • SLUG Antibody (YA3542) Product Image
    MedChemExpress Validation

    Flow Cytometry analysis of MCF-7 cells labelling SLUG (red) with SLUG Antibody (anti-SLUG) (HY-P83845). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P807578, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SLUG Antibody (YA3542) Product Image WB
  • SLUG Antibody (YA3542) Product Image FC