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ELOVL5 Antibody (YA1558)

(Synonyms: ELOVL FA elongase 5; Fatty acid elongase 1; hELO1; Very long chain 3-ketoacyl-CoA synthase 5)
Customer Review

Based on 1 Customer Validation

ELOVL5 Antibody (YA1558) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ELOVL5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
  • ELOVL5 Antibody (YA1558) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

ELOVL5 Antibody (YA1558) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ELOVL5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Species Reactivity Database

Entrez Gene: 60481 Human

SwissProt: Q9NYP7 Human

Immunogen

A synthetic peptide of human ELOVL5

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103913

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using ELOVL5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
    Immunocytochemistry analysis of HepG2 cells labeling ELOVL5 with ELOVL5 Antibody (HY-P81813) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELOVL5 Antibody (HY-P81813)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
    Immunocytochemistry analysis of Hela cells labeling ELOVL5 with ELOVL5 Antibody (HY-P81813) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELOVL5 Antibody (HY-P81813) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ELOVL5 encodes an elongase involved in long-chain polyunsaturated fatty acid (LC-PUFA) elongation[1]. Mechanistically, ELOVL5 elongates 18-carbon and 20-carbon PUFA substrates in activated human T cells[2]. In liver models, reduced ELOVL5 activity promotes hepatic steatosis through SREBP-1c activation[3]. Compared with ELOVL2, ELOVL5 shows limited capacity to elongate C22 docosapentaenoic acid toward DHA synthesis[4]. In disease research, ELOVL5 mutations cause spinocerebellar ataxia 38, linking fatty-acid metabolism to cerebellar neurodegeneration[5]. In prostate cancer models, ELOVL5 supports phospholipid elongation and represents a targetable fatty-acid elongase axis[6].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein; Cell projection, dendrite

  • Expression


    Tissue_specificity:Ubiquitous. Highly expressed in the adrenal gland and testis. Weakly expressed in prostate, lung and brain. Expressed in the cerebellum

  • Isoforms & Post-Translational Modification

    Q9NYP7 has 3 isomers: Q9NYP7-1: 35293 Da (predicted); Q9NYP7-2: 38403 Da (predicted); Q9NYP7-3: 9580 Da (predicted).

  • Subunit

    Interacts with TECR

  • SwissProt ID

    Q9NYP7

  • Gene ID
    60481 [NCBI]
  • Synonyms

    ELOVL FA elongase 5; Fatty acid elongase 1; hELO1; Very long chain 3-ketoacyl-CoA synthase 5

  • Research Field

    Cardiovascular

References

[1]. Gizzio J, et al. Defining Active Conformations from Substrate-Bound Structures Enables Active-State AlphaFold2 Modeling of All 437 Human Catalytic Protein Kinase Domains. Biochem J. 2026 Jun 8:BCJ20260137. [Content Brief]

[2]. Robichaud PP, et al. Polyunsaturated fatty acid elongation and desaturation in activated human T-cells: ELOVL5 is the key elongase. J Lipid Res. 2018 Dec;59(12):2383-2396. [Content Brief]

[3]. Lee MJ, et al. Time to HIV rebound after infusion of long-acting broadly neutralising antibodies 3BNC117-LS and 10-1074-LS and analytical treatment interruption (the RIO trial): a double-blind, randomised, placebo-controlled trial. Lancet HIV. 2026 May 27:S2352-3018(26)00059-7. [Content Brief]

[4]. Gregory MK, et al. Molecular basis for differential elongation of omega-3 docosapentaenoic acid by the rat Elovl5 and Elovl2. J Lipid Res. 2013 Oct;54(10):2851-7. [Content Brief]

[5]. Di Gregorio E, et al. ELOVL5 mutations cause spinocerebellar ataxia 38. Am J Hum Genet. 2014 Aug 7;95(2):209-17. [Content Brief]

[6]. Centenera MM, et al. ELOVL5 Is a Critical and Targetable Fatty Acid Elongase in Prostate Cancer. Cancer Res. 2021 Apr 1;81(7):1704-1718. [Content Brief]

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ELOVL5 Antibody (YA1558) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using ELOVL5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling ELOVL5 with ELOVL5 Antibody (HY-P81813) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELOVL5 Antibody (HY-P81813)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ELOVL5 with ELOVL5 Antibody (HY-P81813) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELOVL5 Antibody (HY-P81813) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ELOVL5 Antibody (YA1558) Product Image
    1/16
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
  • ELOVL5 Antibody (YA1558) Product Image
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using ELOVL5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling ELOVL5 with ELOVL5 Antibody (HY-P81813) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELOVL5 Antibody (HY-P81813)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ELOVL5 with ELOVL5 Antibody (HY-P81813) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELOVL5 Antibody (HY-P81813) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
  • Experimental Validation Results for ELOVL5 Antibody (YA1558)
  • ELOVL5 Antibody (YA1558) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using ELOVL5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ELOVL5 Antibody (YA1558) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling ELOVL5 with ELOVL5 Antibody (HY-P81813) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELOVL5 Antibody (HY-P81813)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ELOVL5 Antibody (YA1558) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling ELOVL5 with ELOVL5 Antibody (HY-P81813) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELOVL5 Antibody (HY-P81813) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ELOVL5 Antibody (YA1558) Product Image WB
  • ELOVL5 Antibody (YA1558) Product Image ICC
  • ELOVL5 Antibody (YA1558) Product Image ICC