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PIN1 Antibody (YA1635)

(Synonyms: DOD; UBL5)
Customer Review

Based on 1 Customer Validation

PIN1 Antibody (YA1635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PIN1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PIN1 Antibody (YA1635)
  • Experimental Validation Results for PIN1 Antibody (YA1635)
  • Experimental Validation Results for PIN1 Antibody (YA1635)
  • PIN1 Antibody (YA1635) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

PIN1 Antibody (YA1635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PIN1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 18 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 18 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human Pin1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PIN1 Antibody (YA1635)
    Western blot analysis of extracts from HEK-293(lane 2(20μg)), HeLa(lane 3(20μg)), NIH-3T3(lane 4(20μg)) and Raji(lane 5(20μg)) using PIN1 Antibody（HY-P81890）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PIN1 Antibody (YA1635)
    Immunocytochemistry analysis of HeLa cells labeling PIN1 with PIN1 Antibody (HY-P81890) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PIN1 Antibody (HY-P81890) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PIN1 Antibody (YA1635)
    Immunocytochemistry analysis of NIH/3T3 cells labeling PIN1 with PIN1 Antibody (HY-P81890) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PIN1 Antibody (HY-P81890) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PIN1 is a peptidyl-prolyl isomerase (PPIase) that specifically recognizes the pSer/Thr-Pro motif, catalyzes the cis and trans isomerization of the proline amide bond, changes the conformation of the substrate protein, thereby affecting its function, stability or intracellular localization. PIN1 regulates cell cycle proteins such as cyclin D, cyclin E, and CDC25, thereby regulating the G1-S process and mitotic progression. PIN1 regulates the stability of proteins such as p53 and p73, responds to stress signals such as DNA damage, and thus affects cell cycle arrest or apoptosis. PIN1 regulates the phosphorylation of tau protein, mediating the neurological functions. PIN1 regulates transcription factors such as NF-κB and JUN, mediates immune responses. PIN1 dysfunction could lead to Alzheimer's disease, cancer, asthma, or microbial infection[1][2].

  • Subcellular Localization

    Nucleus; Nucleus speckle; Cytoplasm

  • Expression


    Tissue_specificity:Expressed in immune cells in the lung (at protein level) (PubMed:29686383) . The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells

  • Subunit

    Interacts with STIL (By similarity). Interacts with KIF20B (PubMed:11470801). Interacts with NEK6 (PubMed:16476580). Interacts (via WW domain) with PRKX (PubMed:19367327). Interacts with BTK (PubMed:16644721). Interacts (via PpiC domain) with DAPK1 (PubMed:21497122). Interacts with the phosphorylated form of RAF1 (PubMed:15664191). Interacts (via WW domain) with ATCAY; upon NGF stimulation (PubMed:18628984). Interacts with PML (isoform PML-4) (PubMed:22033920). Interacts with BCL6 (PubMed:17828269). Interacts with FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923). Directly interacts with RBBP8/CtIP; this interaction depends upon RBBP8 phosphorylation (PubMed:23623683). Interacts (via WW domain) with IRAK3/IRAK-M (when phosphorylated at 'Ser-110') in response to IL33-mediated (but not TLR4 ligand LPS) dendritic cell stimulation (PubMed:29686383). Interacts with PGK1 (when phosphorylated at 'Ser-203'); the interaction is direct, occurs under hypoxic conditions, and targets PGK1 to the mitochondrion by promoting interactions with the TOM complex (PubMed:26942675)

  • SwissProt ID

    Q13526

  • Gene ID
    5300 [NCBI]
  • Synonyms

    DOD; UBL5

  • Research Field

    Neuroscience

References

[1]. Yeh ES, et al., PIN1, the cell cycle and cancer. Nat Rev Cancer. 2007 May;7(5):381-8. [Content Brief]

[2]. Lu KP, et al., The prolyl isomerase PIN1: a pivotal new twist in phosphorylation signalling and disease. Nat Rev Mol Cell Biol. 2007 Nov;8(11):904-16. [Content Brief]

PIN1 Antibody (YA1635) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK-293(lane 2(20μg)), HeLa(lane 3(20μg)), NIH-3T3(lane 4(20μg)) and Raji(lane 5(20μg)) using PIN1 Antibody（HY-P81890）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling PIN1 with PIN1 Antibody (HY-P81890) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PIN1 Antibody (HY-P81890) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH/3T3 cells labeling PIN1 with PIN1 Antibody (HY-P81890) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PIN1 Antibody (HY-P81890) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PIN1 Antibody (YA1635) Product Image
    1/16
  • Experimental Validation Results for PIN1 Antibody (YA1635)
  • Experimental Validation Results for PIN1 Antibody (YA1635)
  • Experimental Validation Results for PIN1 Antibody (YA1635)
  • PIN1 Antibody (YA1635) Product Image
  • Experimental Validation Results for PIN1 Antibody (YA1635)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK-293(lane 2(20μg)), HeLa(lane 3(20μg)), NIH-3T3(lane 4(20μg)) and Raji(lane 5(20μg)) using PIN1 Antibody（HY-P81890）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PIN1 Antibody (YA1635)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling PIN1 with PIN1 Antibody (HY-P81890) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PIN1 Antibody (HY-P81890) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PIN1 Antibody (YA1635)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH/3T3 cells labeling PIN1 with PIN1 Antibody (HY-P81890) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PIN1 Antibody (HY-P81890) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PIN1 Antibody (YA1635)
  • Experimental Validation Results for PIN1 Antibody (YA1635)
  • Experimental Validation Results for PIN1 Antibody (YA1635)
  • PIN1 Antibody (YA1635) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK-293(lane 2(20μg)), HeLa(lane 3(20μg)), NIH-3T3(lane 4(20μg)) and Raji(lane 5(20μg)) using PIN1 Antibody（HY-P81890）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • PIN1 Antibody (YA1635) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling PIN1 with PIN1 Antibody (HY-P81890) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PIN1 Antibody (HY-P81890) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PIN1 Antibody (YA1635) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH/3T3 cells labeling PIN1 with PIN1 Antibody (HY-P81890) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PIN1 Antibody (HY-P81890) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PIN1 Antibody (YA1635) Product Image WB
  • PIN1 Antibody (YA1635) Product Image ICC
  • PIN1 Antibody (YA1635) Product Image ICC