PIN1 Antibody (YA1635)

製品番号: HY-P81890: データシート SDS COA User Guide for Antibodies
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PIN1 Antibody (YA1635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PIN1.

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容量	価格（税別）	在庫状況	数量
20 μL	$146	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

PIN1 Antibody (YA1635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PIN1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 18 kDa;

Observed band size: 18 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13526

Gene ID

5300 [NCBI]

Immunogen

A synthetic peptide of human Pin1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:20

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from HEK-293(lane 2(20μg)), HeLa(lane 3(20μg)), NIH-3T3(lane 4(20μg)) and Raji(lane 5(20μg)) using PIN1 Antibody（HY-P81890）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling PIN1 with PIN1 Antibody (HY-P81890) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PIN1 Antibody (HY-P81890) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of NIH/3T3 cells labeling PIN1 with PIN1 Antibody (HY-P81890) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PIN1 Antibody (HY-P81890) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs (PubMed:21497122, PubMed:23623683, PubMed:29686383). By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes (PubMed:21497122, PubMed:22033920, PubMed:23623683). Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923). May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:23623683, PubMed:27561354). Upon IL33-induced lung inflammation, catalyzes cis-trans isomerization of phosphorylated IRAK3/IRAK-M, inducing IRAK3 stabilization, nuclear translocation and expression of pro-inflammatory genes in dendritic cells (PubMed:29686383). Catalyzes cis-trans isomerization of phosphorylated phosphoglycerate kinase PGK1 under hypoxic conditions to promote its binding to the TOM complex and targeting to the mitochondrion (PubMed:26942675)

Subcellular Localization：Nucleus; Nucleus speckle; Cytoplasm

Expression：
Tissue_specificity:Expressed in immune cells in the lung (at protein level) (PubMed:29686383) . The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells

Subunit：Interacts with STIL (By similarity). Interacts with KIF20B (PubMed:11470801). Interacts with NEK6 (PubMed:16476580). Interacts (via WW domain) with PRKX (PubMed:19367327). Interacts with BTK (PubMed:16644721). Interacts (via PpiC domain) with DAPK1 (PubMed:21497122). Interacts with the phosphorylated form of RAF1 (PubMed:15664191). Interacts (via WW domain) with ATCAY; upon NGF stimulation (PubMed:18628984). Interacts with PML (isoform PML-4) (PubMed:22033920). Interacts with BCL6 (PubMed:17828269). Interacts with FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923). Directly interacts with RBBP8/CtIP; this interaction depends upon RBBP8 phosphorylation (PubMed:23623683). Interacts (via WW domain) with IRAK3/IRAK-M (when phosphorylated at 'Ser-110') in response to IL33-mediated (but not TLR4 ligand LPS) dendritic cell stimulation (PubMed:29686383). Interacts with PGK1 (when phosphorylated at 'Ser-203'); the interaction is direct, occurs under hypoxic conditions, and targets PGK1 to the mitochondrion by promoting interactions with the TOM complex (PubMed:26942675)

Database

Entrez Gene: 5300 Human ; 23988 Mouse ; 298696 Rat

SwissProt: Q13526 Human ; Q9QUR7 Mouse ;

OMIM: 601052 Human

Research Field

Neuroscience

Synonyms

DOD; UBL5

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/a549-cell.html [Content Brief]