Copy Link Print Information

Cyclin D1/CCND1 Antibody (YA4806)

(Synonyms: CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene)
Customer Review

Based on 1 Customer Validation

Cyclin D1/CCND1 Antibody (YA4806) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cyclin D1/CCND1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA4806)
  • Cyclin D1/CCND1 Antibody (YA4806) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:10000

Product Details

Description

Cyclin D1/CCND1 Antibody (YA4806) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cyclin D1/CCND1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 34 kDa;
Immunogen

Purified recombinant fragment of human Cyclin D1 expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

RRID

AB_3719017

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA4806)
    Western blot analysis of extracts from MCF7(lane 2(40μg), A431(lane 3(40μg), SH-SY5Y(lane 4(40μg),HCT116(lane 5(40μg)using Cyclin D1/CCND1 Antibody (YA4806). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

Background

  • Function

    Cyclin D1/CCND1 is a Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Nucleus; Cytoplasm; Nucleus membrane

  • Subunit

    Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex (PubMed:19237565, PubMed:8114739). The cyclin subunit imparts substrate specificity to the complex (PubMed:19237565, PubMed:20399237, PubMed:8302605, PubMed:9106657). Component of the ternary complex CCND1/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity (PubMed:9106657). Interacts directly with CDKN1B (By similarity). Can form similar complexes with either CDKN1A or CDKN2A (By similarity). Interacts with UHRF2; the interaction ubiquitinates CCND1 and appears to occur independently of phosphorylation (PubMed:21952639). Interacts with USP2 (PubMed:19917254). Interacts (via cyclin N-terminal domain) with INSM1 (via N-terminal region); the interaction competes with the binding of CCND1 to CDK4 during cell cycle progression and inhibits CDK4 activity (PubMed:16569215, PubMed:18417529, PubMed:19124461). Interacts with CDK4; the interaction is prevented with the binding of CCND1 to INSM1 during cell cycle progression (PubMed:19124461)

  • SwissProt ID

    P24385

  • Gene ID
    595 [NCBI]
  • Synonyms

    CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene

Cyclin D1/CCND1 Antibody (YA4806) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF7(lane 2(40μg), A431(lane 3(40μg), SH-SY5Y(lane 4(40μg),HCT116(lane 5(40μg)using Cyclin D1/CCND1 Antibody (YA4806). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

  • Cyclin D1/CCND1 Antibody (YA4806) Product Image
    1/16
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA4806)
  • Cyclin D1/CCND1 Antibody (YA4806) Product Image
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA4806)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF7(lane 2(40μg), A431(lane 3(40μg), SH-SY5Y(lane 4(40μg),HCT116(lane 5(40μg)using Cyclin D1/CCND1 Antibody (YA4806). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA4806)
  • Cyclin D1/CCND1 Antibody (YA4806) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF7(lane 2(40μg), A431(lane 3(40μg), SH-SY5Y(lane 4(40μg),HCT116(lane 5(40μg)using Cyclin D1/CCND1 Antibody (YA4806). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

  • Cyclin D1/CCND1 Antibody (YA4806) Product Image WB