ZEB1 Antibody (YA6252)

Cat. No.: HY-P86560: Data Sheet COA
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ZEB1 Antibody (YA6252) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZEB1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ZEB1 Antibody (YA6252) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZEB1.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 124 kDa;

Observed band size: 200 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P37275

Gene ID

6935 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:500
WB WB: Western Blot	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG/Kappa

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:In established cell lines, SMARCA4/BRG1 is colocalized (at the protein level) with E-cadherin-negative cells, normal colonic stromal, and dedifferentiated epithelial cells at the invasive front of colorectal cancer. It is expressed in the heart and skeletal muscle, but not in the liver, spleen, or pancreas.

Isoforms & Post-Translational Modification：P37275 has 5 isomers: P37275-1: 124074 Da (predicted); P37275-2: 124076 Da (predicted); P37275-3: 116593 Da (predicted); P37275-4: 122003 Da (predicted); P37275-5: 122084 Da (predicted).
Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization

Subunit：Interacts (via N-terminus) with SMARCA4/BRG1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)