ZEB1 Antibody (YA6252)
Based on 1 Customer Validation
ZEB1 Antibody (YA6252) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZEB1.
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Host:
Rabbit
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Isotype:
IgG/Kappa
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Application:
WB, IHC-P, ICC/IF, ELISA
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Reactivity :
Human, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1:500 | 1:1000-1:5000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
ZEB1 Antibody (YA6252) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZEB1.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 200 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 124 kDa
Protein A
Non-conjugated
Unmodified
IgG/Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ZEB1 acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:In established cell lines, SMARCA4/BRG1 is colocalized (at the protein level) with E-cadherin-negative cells, normal colonic stromal, and dedifferentiated epithelial cells at the invasive front of colorectal cancer. It is expressed in the heart and skeletal muscle, but not in the liver, spleen, or pancreas. -
Isoforms & Post-Translational Modification
P37275 has 5 isomers: P37275-1: 124074 Da (predicted); P37275-2: 124076 Da (predicted); P37275-3: 116593 Da (predicted); P37275-4: 122003 Da (predicted); P37275-5: 122084 Da (predicted).
Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization -
Subunit
Interacts (via N-terminus) with SMARCA4/BRG1
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SwissProt ID
Documentation
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Data Sheet (234 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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User Guide for Antibodies (1077 KB)
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