SESN1 Antibody (YA9742)
(Synonyms: 1110002G11Rik, AU044290, MGC118148, MGC138241, MGC142129, OTTMUSP00000022799, p53 activated gene 26, p53 regulated PA26 nuclear protein, p53 regulated protein PA26, p53-regulated protein PA26)SESN1 Antibody (YA9742) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to SESN1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IHC-P, FC
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Reactivity :
Human
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Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:100 | 1:1000 | 1:1000 |
Product Details
SESN1 Antibody (YA9742) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to SESN1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 57 kDa;
Synthetic peptide of Human SESN1 (aa 100-200).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SESN1 functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway. This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1. Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1. However, this could not be confirmed (By similarity)[1][2][3][4].
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_Specificity: Widely expressed.
Induction: Isoform T2 and isoform T3 are induced by genotoxic stress (UV, gamma-irradiation and cytotoxic drugs) in a p53/TP53-dependent manner. Isoform T1 is not induced by p53/TP53. -
Isoforms & Post-Translational Modification
SESN1 has 3 isoforms, Q9Y6P5-1: amino acid length is 492, molecular weight is 56557 Da (predicted); Q9Y6P5-2: amino acid length is 551, molecular weight is 63826 Da (predicted); Q9Y6P5-3: amino acid length is 426, molecular weight is 49624 Da (predicted).
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Subunit
Interacts with the GATOR2 complex which is composed of MIOS, SEC13, SEH1L, WDR24 and WDR59; the interaction is negatively regulated by leucine.
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SwissProt ID
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Synonyms
1110002G11Rik, AU044290, MGC118148, MGC138241, MGC142129, OTTMUSP00000022799, p53 activated gene 26, p53 regulated PA26 nuclear protein, p53 regulated protein PA26, p53-regulated protein PA26
Documentation
References
[1]. Chantranupong L, et al. The Sestrins interact with GATOR2 to negatively regulate the amino-acid-sensing pathway upstream of mTORC1. Cell Rep. 2014 Oct 9;9(1):1-8. [Content Brief]
[2]. Wolfson RL, et al. Sestrin2 is a leucine sensor for the mTORC1 pathway. Science. 2016 Jan 1;351(6268):43-8. [Content Brief]
[3]. Bae SH, et al. Sestrins activate Nrf2 by promoting p62-dependent autophagic degradation of Keap1 and prevent oxidative liver damage. Cell Metab. 2013 Jan 8;17(1):73-84. [Content Brief]
[4]. Budanov AV, et al. Regeneration of peroxiredoxins by p53-regulated sestrins, homologs of bacterial AhpD. Science. 2004 Apr 23;304(5670):596-600. [Content Brief]
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