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Lamin B1 Antibody (YA313)

(Synonyms: Lamin-B1)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

Lamin B1 Antibody (YA313) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lamin B1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

ALL WB IHC-P ICC
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Lamin B1 Antibody (YA313) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

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Publications Citing Use of MedChemExpress (MCE) Lamin B1 Antibody (YA313)

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2 Publications Citing Use of MCE Lamin B1 Antibody (YA313) (1)
WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:50,000 1:500-1:1,000 1:500-1:1,000

Product Details

Description

Lamin B1 Antibody (YA313) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lamin B1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 66 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 66 kDa
Immunogen

Synthetic peptide within Human Lamin B1 aa 511-560 / 586.

Purification

Protein A affinity purified.

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Lamin B1 Antibody (YA313)
    Western blot analysis of extracts from HEK293 (lane 2(20μg), Hela (lane 3(20μg) and THP-1(lane 4(20μg) using Lamin B1(HY-P80205) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Lamin B1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80205, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Lamin B1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80205, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
    Immunocytochemistry analysis of HepG2 cells labeling Lamin B1 with Lamin B1 Antibody (HY-P80205)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lamin B1 Antibody (HY-P80205) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
    Immunocytochemistry analysis of HepG2 cells labeling Lamin B1 with Lamin B1 Antibody (HY-P80205) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lamin B1 Antibody (HY-P80205) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Lamin B1 is a Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively[1][2].

  • Subcellular Localization

    Nucleus lamina

  • Subunit

    Homodimer (PubMed:22265972, PubMed:33706103). Lamin dimers then assemble into dimeric head-to-tail polymers.

  • SwissProt ID

    P20700

  • Gene ID
    4001 [NCBI]
  • Synonyms

    Lamin-B1

[1]. Pedroso JL, et al. LMNB1 mutation causes cerebellar involvement and a genome instability defect. J Neurol Sci. 2017 Aug 15;379:249-252. [Content Brief]

[2]. Cristofoli F, et al. De Novo Variants in LMNB1 Cause Pronounced Syndromic Microcephaly and Disruption of Nuclear Envelope Integrity. Am J Hum Genet. 2020 Oct 1;107(4):753-762. [Content Brief]

Lamin B1 Antibody (YA313) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), Hela (lane 3(20μg) and THP-1(lane 4(20μg) using Lamin B1(HY-P80205) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Lamin B1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80205, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Lamin B1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80205, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Lamin B1 with Lamin B1 Antibody (HY-P80205)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lamin B1 Antibody (HY-P80205) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Lamin B1 with Lamin B1 Antibody (HY-P80205) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lamin B1 Antibody (HY-P80205) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    Lamin B1 Antibody (YA313) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Oct;11(39):e2309754. [Abstract]

    Customer Validation
     Western blotting analysis of renal Meis1 in cytoplasm and nucleus in UUO and UIRI models. Lamin B1 and GAPDH was used as loading control (n = 3 in each group).

  • Lamin B1 Antibody (YA313) Product Image
    1/16
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Lamin B1 Antibody (YA313) Product Image
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), Hela (lane 3(20μg) and THP-1(lane 4(20μg) using Lamin B1(HY-P80205) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Lamin B1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80205, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Lamin B1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80205, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Lamin B1 with Lamin B1 Antibody (HY-P80205)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lamin B1 Antibody (HY-P80205) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Lamin B1 with Lamin B1 Antibody (HY-P80205) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lamin B1 Antibody (HY-P80205) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)
  • Experimental Validation Results for Lamin B1 Antibody (YA313)

  • Lamin B1 Antibody (YA313) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Oct;11(39):e2309754. [Abstract]

    Western blotting analysis of renal Meis1 in cytoplasm and nucleus in UUO and UIRI models. Lamin B1 and GAPDH was used as loading control (n = 3 in each group).

  • Lamin B1 Antibody (YA313) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2(20μg), Hela (lane 3(20μg) and THP-1(lane 4(20μg) using Lamin B1(HY-P80205) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Lamin B1 Antibody (YA313) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Lamin B1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80205, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lamin B1 Antibody (YA313) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Lamin B1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80205, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lamin B1 Antibody (YA313) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Lamin B1 with Lamin B1 Antibody (HY-P80205)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lamin B1 Antibody (HY-P80205) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Lamin B1 Antibody (YA313) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Lamin B1 with Lamin B1 Antibody (HY-P80205) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lamin B1 Antibody (HY-P80205) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Lamin B1 Antibody (YA313) Product Image WB
  • Lamin B1 Antibody (YA313) Product Image IHC-P
  • Lamin B1 Antibody (YA313) Product Image IHC-P
  • Lamin B1 Antibody (YA313) Product Image ICC
  • Lamin B1 Antibody (YA313) Product Image ICC