Copy Link Print Information

CDKN2A/p16INK4a Antibody (YA3494)

(Synonyms: ARF antibody; CDK4 inhibitor p16 INK4 antibody; CDK4I antibody; CDKN2 antibody; CDKN2A antibody; Cell cycle negative regulator beta antibody; CMM2 antibody; INK4 antibody; INK4a antibody; Melanoma p16 inhibits CDK4 antibody; MLM antibody; MTS1 antibody; Multiple tumor suppressor 1 antibody; p16 antibody; p16 INK4a antibody; p16INK4a antibody; p19 antibody; P19ARF antibody; TP16 antibody; )
Customer Review

Based on 1 Customer Validation

CDKN2A/p16INK4a Antibody (YA3494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA3494)
  • CDKN2A/p16INK4a Antibody (YA3494) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:1000

Product Details

Description

CDKN2A/p16INK4a Antibody (YA3494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.

  • Host Rabbit
  • Clonality Monoclonal, Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Species Reactivity Database

Entrez Gene: 1029 Human

SwissProt: P42771 Human

Immunogen

Synthetic peptide.

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102786

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA3494)
    Western blot analysis of extracts from Hela(lane 2(20μg), HEK293 (lane 3(20μg) and HepG2 (lane 4(20μg) using CDKN2A/p16INK4a (HY-P80992) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:It is widely expressed, but not detected in the brain or skeletal muscle. Isomer 3 is pancreas-specific.
    Positive sample: HeLa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH:OVCAR-3 cell lysate, human ovary carcinoma tissue.

  • Isoforms & Post-Translational Modification

    P42771 has 6 isomers: P42771-1: 16533 Da (predicted); P42771-2: 11215 Da (predicted); P42771-3: 12083 Da (predicted); Q8N726-1: 13903 Da (predicted); P42771-4: 17883 Da (predicted); Q8N726-2: 8731 Da (predicted).
    Phosphorylation seems to increase interaction with CDK4

  • Subunit

    Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2

  • SwissProt ID

    P42771

  • Gene ID
    1029 [NCBI]
  • Synonyms

    ARF antibody; CDK4 inhibitor p16 INK4 antibody; CDK4I antibody; CDKN2 antibody; CDKN2A antibody; Cell cycle negative regulator beta antibody; CMM2 antibody; INK4 antibody; INK4a antibody; Melanoma p16 inhibits CDK4 antibody; MLM antibody; MTS1 antibody; Multiple tumor suppressor 1 antibody; p16 antibody; p16 INK4a antibody; p16INK4a antibody; p19 antibody; P19ARF antibody; TP16 antibody;

CDKN2A/p16INK4a Antibody (YA3494) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg), HEK293 (lane 3(20μg) and HepG2 (lane 4(20μg) using CDKN2A/p16INK4a (HY-P80992) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CDKN2A/p16INK4a Antibody (YA3494) Product Image
    1/16
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA3494)
  • CDKN2A/p16INK4a Antibody (YA3494) Product Image
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA3494)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg), HEK293 (lane 3(20μg) and HepG2 (lane 4(20μg) using CDKN2A/p16INK4a (HY-P80992) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA3494)
  • CDKN2A/p16INK4a Antibody (YA3494) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg), HEK293 (lane 3(20μg) and HepG2 (lane 4(20μg) using CDKN2A/p16INK4a (HY-P80992) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CDKN2A/p16INK4a Antibody (YA3494) Product Image WB