CDKN2A/p16INK4a Antibody (YA3494)

Cat. No.: HY-P80992: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

CDKN2A/p16INK4a Antibody (YA3494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CDKN2A/p16INK4a Antibody (YA3494) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CDKN2A/p16INK4a Antibody (YA3494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molecular Weight

Predicted band size: 17 kDa;

Observed band size: 17 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P42771

Gene ID

1029 [NCBI]

Immunogen

Synthetic peptide.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000

Sensitivity	Endogenous	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela(lane 2(20μg), HEK293 (lane 3(20μg) and HepG2 (lane 4(20μg) using CDKN2A/p16INK4a (HY-P80992) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:It is widely expressed, but not detected in the brain or skeletal muscle. Isomer 3 is pancreas-specific.
Positive sample: HeLa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH:OVCAR-3 cell lysate, human ovary carcinoma tissue.

Isoforms & Post-Translational Modification：P42771 has 6 isomers: P42771-1: 16533 Da (predicted); P42771-2: 11215 Da (predicted); P42771-3: 12083 Da (predicted); Q8N726-1: 13903 Da (predicted); P42771-4: 17883 Da (predicted); Q8N726-2: 8731 Da (predicted).
Phosphorylation seems to increase interaction with CDK4

Subunit：Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2

RRID

AB_3102786

Database

Entrez Gene: 1029 Human

SwissProt: P42771 Human

OMIM: 155601 Human

Synonyms

ARF antibody; CDK4 inhibitor p16 INK4 antibody; CDK4I antibody; CDKN2 antibody; CDKN2A antibody; Cell cycle negative regulator beta antibody; CMM2 antibody; INK4 antibody; INK4a antibody; Melanoma p16 inhibits CDK4 antibody; MLM antibody; MTS1 antibody; Multiple tumor suppressor 1 antibody; p16 antibody; p16 INK4a antibody; p16INK4a antibody; p19 antibody; P19ARF antibody; TP16 antibody;

Documentation

Select Batch:

Data Sheet (232 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

CDKN2A/p16INK4a Antibody (YA3494) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CDKN2A/p16INK4a Antibody (YA3494)

CDKN2A/p16INK4a Antibody (YA3494) Featured Recommendations:

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