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FKBP12 Antibody (YA434)

(Synonyms: FKBP1, FKBP12, FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, PPIase FKBP1A, 12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, Immunophilin FKBP12, Rotamase, 12 kDa FKBP, FKBP-12, FKBP-1A)
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Based on 1 Customer Validation

FKBP12 Antibody (YA434) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FKBP12.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FKBP12 Antibody (YA434)
  • FKBP12 Antibody (YA434) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000

Product Details

Description

FKBP12 Antibody (YA434) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FKBP12.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 14 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 12 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human FKBP12.AA range:76-108.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102485

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FKBP12 Antibody (YA434)
    Western blot analysis of extracts from SH-SY5Y(lane 2(20μg) and SH-SY5Y(lane 3(40μg), U937(lane 4(20μg) ) and U937(lane 5(40μg) ) using FKBP12 (HY-P80400) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80400, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    FKBP12 (FKBP1A) is a highly conserved immunophilin that functions as a peptidyl-prolyl cis-trans isomerase and participates in protein folding, intracellular signaling, and calcium homeostasis[1]. Mechanistically, FKBP12 forms regulatory complexes with multiple signaling proteins, including ryanodine receptors, transforming growth factor-β (TGF-β) type I receptors, and the mammalian target of rapamycin (mTOR)， thereby linking protein conformational control to signal transduction pathways[1][2]. A key biological process involving FKBP12 is the regulation of intracellular Ca2+ release, where FKBP12 associates with ryanodine receptor channels and contributes to channel gating and calcium signaling dynamics[1][3]. Through its interaction with immunosuppressive ligands, FKBP12 also serves as an essential component of the calcineurin and mTOR signaling pathways; the FKBP12-FK506 complex inhibits calcineurin, whereas the FKBP12-rapamycin complex binds the FKBP-rapamycin-binding domain of mTOR and suppresses mTOR-dependent cellular growth signaling[1][4]. Disease relevance is supported by genetic studies showing that FKBP12 deficiency in mice causes embryonic lethality associated with severe cardiac defects, highlighting its importance in cardiovascular development and calcium signaling regulation[1]. Compared with the closely related isoform FKBP12.6 (FKBP1B)， FKBP12 shares structural similarity and regulatory activity toward ryanodine receptors, but FKBP12.6 exhibits distinct channel-specific interactions, particularly in cardiac calcium release systems[1]. For experimental applications, FK506 (tacrolimus) and rapamycin remain the most widely used FKBP12-binding ligands and serve as valuable chemical tools for investigating calcineurin signaling, mTOR regulation, and protein-protein interaction networks[1][4].

  • Subcellular Localization

    Cytoplasm, cytosol; Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side

  • Subunit

    Interacts with TGFBR1; prevents TGFBR1 phosphorylation by TGFBR2 and stabilizes it in the inactive conformation (PubMed:9233797). Interacts with ACVR1B and SMAD7 (PubMed:16720724).

  • SwissProt ID

    P62942

  • Gene ID
    2280 [NCBI]
  • Synonyms

    FKBP1, FKBP12, FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, PPIase FKBP1A, 12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, Immunophilin FKBP12, Rotamase, 12 kDa FKBP, FKBP-12, FKBP-1A

  • Research Field

    Immunology

[1]. Tong M, et al. FK506-Binding Proteins and Their Diverse Functions. Curr Mol Pharmacol. 2015;9(1):48-65. [Content Brief]

[2]. Hoeffer CA, et al. Removal of FKBP12 enhances mTOR-Raptor interactions, LTP, memory, and perseverative/repetitive behavior. Neuron. 2008 Dec 10;60(5):832-45. doi: 10.1016/j.neuron.2008.09.037. PMID: 19081378; PMCID: PMC2630531.

[3]. Carmody M, et al. FKBP12 associates tightly with the skeletal muscle type 1 ryanodine receptor, but not with other intracellular calcium release channels. FEBS Lett. 2001 Sep 7;505(1):97-102. [Content Brief]

[4]. Kolos JM, et al. FKBP Ligands-Where We Are and Where to Go? Front Pharmacol. 2018 Dec 5;9:1425. [Content Brief]

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FKBP12 Antibody (YA434) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20μg) and SH-SY5Y(lane 3(40μg), U937(lane 4(20μg) ) and U937(lane 5(40μg) ) using FKBP12 (HY-P80400) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80400, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • FKBP12 Antibody (YA434) Product Image
    1/16
  • Experimental Validation Results for FKBP12 Antibody (YA434)
  • FKBP12 Antibody (YA434) Product Image
  • Experimental Validation Results for FKBP12 Antibody (YA434)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20μg) and SH-SY5Y(lane 3(40μg), U937(lane 4(20μg) ) and U937(lane 5(40μg) ) using FKBP12 (HY-P80400) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80400, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FKBP12 Antibody (YA434)
  • FKBP12 Antibody (YA434) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from SH-SY5Y(lane 2(20μg) and SH-SY5Y(lane 3(40μg), U937(lane 4(20μg) ) and U937(lane 5(40μg) ) using FKBP12 (HY-P80400) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80400, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • FKBP12 Antibody (YA434) Product Image WB