Copy Link Print Information

GWL Antibody (YA6585)

(Synonyms: 2700091H24Rik C88295 FLJ14813 GREATWALL Greatwall protein kinase GW GWL GWL_HUMAN hGWL MAST-L 2700091H24Rik C88295 FLJ14813 GREATWALL Greatwall protein kinase GW GWL GWL_HUMAN hGWL MAST-L Mastl MGC117975 Microtubule associated serine/threonine kinase like Microtubule-associated serine/threonine-protein kinase-like RP11 85G18.2 Serine/threonine-protein kinase greatwall THC2 )

GWL Antibody (YA6585) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GWL.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GWL Antibody (YA6585)
  • GWL Antibody (YA6585) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1:50 1-2μg/sample

Product Details

Description

GWL Antibody (YA6585) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GWL.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Monkey
  • Observed Molecular Weight
    Observed band size: 110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 97 kDa
Immunogen

Recombinant protein within human GWL aa 1-350.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GWL Antibody (YA6585)
    Western blot analysis of extracts from Hela (lane2, 20μg), LNCap (lane3, 20μg), HepG2 (lane4, 20μg) and Jurkat (lane5, 20μg) using GWL Antibody (HY-P86892). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    GWL is a Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation[1][2][3].

  • Subcellular Localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Nucleus,Cleavage furrow

  • Isoforms & Post-Translational Modification

    Q96GX5 has three isomers: Q96GX5-1: 97319 Da (predicted); Q96GX5-2: 92861 Da (predicted); Q96GX5-3: 97191 Da (predicted).
    Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity)

  • SwissProt ID

    Q96GX5

  • Gene ID
    84930 [NCBI]
  • Synonyms

    2700091H24Rik C88295 FLJ14813 GREATWALL Greatwall protein kinase GW GWL GWL_HUMAN hGWL MAST-L 2700091H24Rik C88295 FLJ14813 GREATWALL Greatwall protein kinase GW GWL GWL_HUMAN hGWL MAST-L Mastl MGC117975 Microtubule associated serine/threonine kinase like Microtubule-associated serine/threonine-protein kinase-like RP11 85G18.2 Serine/threonine-protein kinase greatwall THC2

GWL Antibody (YA6585) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), LNCap (lane3, 20μg), HepG2 (lane4, 20μg) and Jurkat (lane5, 20μg) using GWL Antibody (HY-P86892). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • GWL Antibody (YA6585) Product Image
    1/16
  • Experimental Validation Results for GWL Antibody (YA6585)
  • GWL Antibody (YA6585) Product Image
  • Experimental Validation Results for GWL Antibody (YA6585)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), LNCap (lane3, 20μg), HepG2 (lane4, 20μg) and Jurkat (lane5, 20μg) using GWL Antibody (HY-P86892). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GWL Antibody (YA6585)
  • GWL Antibody (YA6585) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), LNCap (lane3, 20μg), HepG2 (lane4, 20μg) and Jurkat (lane5, 20μg) using GWL Antibody (HY-P86892). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • GWL Antibody (YA6585) Product Image WB