GWL Antibody (YA6585)

Cat. No.: HY-P86892: Data Sheet User Guide for Antibodies
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GWL Antibody (YA6585) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GWL.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GWL Antibody (YA6585) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GWL.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 97 kDa;

Observed band size: 110 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Monkey

SwissProt ID

Q96GX5

Gene ID

84930 [NCBI]

Immunogen

Recombinant protein within human GWL aa 1-350.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50
IP IP: Immunoprecipitation	1-2μg/sample

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance (PubMed:19680222). Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively (PubMed:38123684). ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high (PubMed:20818157). Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation

Subcellular Localization：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Nucleus,Cleavage furrow

Isoforms & Post-Translational Modification：Q96GX5 has three isomers: Q96GX5-1: 97319 Da (predicted); Q96GX5-2: 92861 Da (predicted); Q96GX5-3: 97191 Da (predicted).
Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity)

Synonyms

2700091H24Rik C88295 FLJ14813 GREATWALL Greatwall protein kinase GW GWL GWL_HUMAN hGWL MAST-L 2700091H24Rik C88295 FLJ14813 GREATWALL Greatwall protein kinase GW GWL GWL_HUMAN hGWL MAST-L Mastl MGC117975 Microtubule associated serine/threonine kinase like Microtubule-associated serine/threonine-protein kinase-like RP11 85G18.2 Serine/threonine-protein kinase greatwall THC2

Documentation

User Guide for Antibodies (1077 KB)