Factor X Antibody (YA1349)
(Synonyms: Activated factor Xa heavy chain; Coagulation factor X; F10; factor Xa; FXA; Prothrombinase; Stuart factor)
Based on 1 Customer Validation
Factor X Antibody (YA1349) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Factor X.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human
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Formulation:
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:50-1:100 |
Product Details
Factor X Antibody (YA1349) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Factor X.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 74 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 55 kDa
A synthesized peptide derived from human Factor X aa150-300/488.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Factor X is a Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner. Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells. Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner. Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues. Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues. Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues. Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues. Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism. Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa. Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues[1][2][3][4][5][6][7].
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Subcellular Localization
Secreted
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Expression
Tissue_specificity:Plasma; synthesized in the liver -
Subunit
The two chains are formed from a single-chain precursor by the excision of two Arg residues and are held together by 1 or more disulfide bonds. Forms a heterodimer with SERPINA5. Interacts (inactive and activated) with ixolaris, an anticoagulant protein from Ixodes scapularis saliva (PubMed:11986214, PubMed:31133602, PubMed:18042685, PubMed:16807644). Interacts (activated) with iripin-8, a serine protease inhibitor from Ixodes ricinus saliva (PubMed:34502392). Interacts (activated) with FXa-directed anticoagulant from Aedes albopictus saliva (PubMed:21673107). Interacts (activated) with guianensin, an anticoagulant protein from Simulium guianense saliva (PubMed:37469515). Interacts (activated) with simukunin, an anticoagulant protein from Simulium vittatum saliva (PubMed:22383955)
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SwissProt ID
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Synonyms
Activated factor Xa heavy chain; Coagulation factor X; F10; factor Xa; FXA; Prothrombinase; Stuart factor
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Research Field
Cardiovascular
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[1]. Manithody C, et al. Identification of exosite residues of factor Xa involved in recognition of PAR-2 on endothelial cells. Biochemistry. 2012 Mar 27;51(12):2551-7. [Content Brief]
[2]. Bukowska A, et al. Coagulation factor Xa induces an inflammatory signalling by activation of protease-activated receptors in human atrial tissue. Eur J Pharmacol. 2013 Oct 15;718(1-3):114-23. [Content Brief]
[3]. Shinozawa E, et al. TAK-442, a Direct Factor Xa Inhibitor, Inhibits Monocyte Chemoattractant Protein 1 Production in Endothelial Cells via Involvement of Protease-Activated Receptor 1. Front Pharmacol. 2018;9:1431. [Content Brief]
[4]. D'Alessandro E, et al. Coagulation Factor Xa Induces Proinflammatory Responses in Cardiac Fibroblasts via Activation of Protease-Activated Receptor-1. Cells. 2021 Oct 30;10(11):. [Content Brief]
[5]. Borensztajn K, et al. Factor Xa stimulates proinflammatory and profibrotic responses in fibroblasts via protease-activated receptor-2 activation. Am J Pathol. 2008 Feb;172(2):309-20. [Content Brief]
[6]. Shrivastava G, et al. Alboserpin, the Main Salivary Anticoagulant from the Disease Vector Aedes albopictus, Displays Anti-FXa-PAR Signaling In Vitro and In Vivo. Immunohorizons. 2022 Jun 23;6(6):373-383. [Content Brief]
[7]. Senden NH, et al. Factor Xa induces cytokine production and expression of adhesion molecules by human umbilical vein endothelial cells. J Immunol. 1998 Oct 15;161(8):4318-24. [Content Brief]
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