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CD161 Antibody (YA853)

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Based on 1 Customer Validation

CD161 Antibody (YA853) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD161.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD161 Antibody (YA853)
  • CD161 Antibody (YA853) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:500 1:500-1:1000

Product Details

Description

CD161 Antibody (YA853) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD161.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Species Reactivity Database

Entrez Gene: 3820 Human

SwissProt: Q12918 Human

Immunogen

Synthetic peptide within human CD161 aa 176-225.

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

RRID

AB_3102795

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD161 Antibody (YA853)
    Western blot analysis of extracts from THP-1(lane 2(20ug) , HL-60(lane 3(20ug) and Jurkat(lane 4(20ug) using CD161 Antibody (HY-P80966) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Also binds to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells

  • Subcellular Localization

    Membrane; Single-pass type II membrane protein

  • Expression


    Tissue_specificity:It is primarily expressed in NK cell subsets in the intestinal epithelium and liver. It can be detected in peripheral blood T cells, especially in adult T cells with a memory antigen phenotype.

    Induction:By IL12/interleukin-12 in NK cells

  • Subunit

    Homodimer; disulfide-linked. Interacts with acid sphingomyelinase/SMPD1

  • SwissProt ID

    Q12918

  • Gene ID
    3820 [NCBI]

CD161 Antibody (YA853) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from THP-1(lane 2(20ug) , HL-60(lane 3(20ug) and Jurkat(lane 4(20ug) using CD161 Antibody (HY-P80966) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD161 Antibody (YA853) Product Image
    1/16
  • Experimental Validation Results for CD161 Antibody (YA853)
  • CD161 Antibody (YA853) Product Image
  • Experimental Validation Results for CD161 Antibody (YA853)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from THP-1(lane 2(20ug) , HL-60(lane 3(20ug) and Jurkat(lane 4(20ug) using CD161 Antibody (HY-P80966) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD161 Antibody (YA853)
  • CD161 Antibody (YA853) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from THP-1(lane 2(20ug) , HL-60(lane 3(20ug) and Jurkat(lane 4(20ug) using CD161 Antibody (HY-P80966) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD161 Antibody (YA853) Product Image WB