H6PD Antibody (YA7845)(PBS only)

Cat. No.: HY-P88161A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

H6PD Antibody (YA7845) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to H6PD.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

H6PD Antibody (YA7845) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to H6PD.

Host

Mouse

Molecular Weight

Predicted band size: 88.7 kDa

Species Reactivity

Human

SwissProt ID

O95479

Immunogen

Full length human recombinant protein of human H6PD produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-250
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Solution

Formulation

Supplied in PBS, pH 7.4.

Shipping

Shipping with blue ice.

Background

Function：Bifunctional enzyme localized in the lumen of the endoplasmic reticulum that catalyzes the first two steps of the oxidative branch of the pentose phosphate pathway/shunt, an alternative to glycolysis and a major source of reducing power and metabolic intermediates for biosynthetic processes (By similarity). Has a hexose-6-phosphate dehydrogenase activity, with broad substrate specificity compared to glucose-6-phosphate 1-dehydrogenase/G6PD, and catalyzes the first step of the pentose phosphate pathway (PubMed:12858176, PubMed:18628520, PubMed:23132696). In addition, acts as a 6-phosphogluconolactonase and catalyzes the second step of the pentose phosphate pathway (By similarity). May have a dehydrogenase activity for alternative substrates including glucosamine 6-phosphate and glucose 6-sulfate (By similarity). The main function of this enzyme is to provide reducing equivalents such as NADPH to maintain the adequate levels of reductive cofactors in the oxidizing environment of the endoplasmic reticulum (PubMed:12858176, PubMed:18628520, PubMed:23132696). By producing NADPH that is needed by reductases of the lumen of the endoplasmic reticulum like corticosteroid 11-beta-dehydrogenase isozyme 1/HSD11B1, indirectly regulates their activity (PubMed:18628520)

Subcellular Localization：Endoplasmic reticulum lumen

Expression：
Tissue_Specificity: Present in most tissues examined, strongest in liver

Isoforms & Post-Translational Modification：O95479 has two isomers: O95479-1: 88893 Da (predicted); O95479-2: 90173 Da (predicted).

Subunit：Homodimer

Synonyms

CORTRD1; G6PDH; GDH

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/huh-7-cell.html [Content Brief]