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MECP2 Antibody (YA4069)

(Synonyms: RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13)
Customer Review

Based on 1 Customer Validation

MECP2 Antibody (YA4069) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MECP2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for MECP2 Antibody (YA4069)
  • Experimental Validation Results for MECP2 Antibody (YA4069)
  • Experimental Validation Results for MECP2 Antibody (YA4069)
  • MECP2 Antibody (YA4069) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

MECP2 Antibody (YA4069) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MECP2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 52 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Immunogen

Purified recombinant fragment of human MECP2 (AA: 7-148) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718892

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MECP2 Antibody (YA4069)
    Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), A549 (lane 4(20μg) and A431 (lane 5(20μg) using MECP2 Antibody (HY-P84372) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MECP2 Antibody (YA4069)
    Western blot analysis of extracts from A431(lane 1(40μg) ) using MECP2 antibody(HY-P84372). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10
  • Experimental Validation Results for MECP2 Antibody (YA4069)
    Flow cytometric analysis of 1X10^6 Hela cells labeling MECP2 Antibody(HY-P84372

Background

  • Function

    MECP2 is a Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC)

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is present in all examined adult somatic tissues.

  • Isoforms & Post-Translational Modification

    P51608 has 2 isomers: P51608-1: 52441 Da (predicted); P51608-2: 53323 Da (predicted).
    Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation

  • Subunit

    Interacts with FNBP3 (By similarity). Interacts with CDKL5 (PubMed:15917271). Interacts with ATRX; MECP2 recruits ATRX to pericentric heterochromatin in neuronal cells (By similarity). Interacts with NCOR2 (By similarity). Interacts with TBL1XR1; bridges interaction between MECP2 and NCOR1 (PubMed:28348241). Interacts with TBL1X; recruits TBL1X to the heterochromatin foci (PubMed:28348241)

  • SwissProt ID

    P51608

  • Gene ID
    4204 [NCBI]
  • Synonyms

    RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13

MECP2 Antibody (YA4069) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), A549 (lane 4(20μg) and A431 (lane 5(20μg) using MECP2 Antibody (HY-P84372) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 1(40μg) ) using MECP2 antibody(HY-P84372). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling MECP2 Antibody(HY-P84372

  • MECP2 Antibody (YA4069) Product Image
    1/16
  • Experimental Validation Results for MECP2 Antibody (YA4069)
  • Experimental Validation Results for MECP2 Antibody (YA4069)
  • Experimental Validation Results for MECP2 Antibody (YA4069)
  • MECP2 Antibody (YA4069) Product Image
  • Experimental Validation Results for MECP2 Antibody (YA4069)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), A549 (lane 4(20μg) and A431 (lane 5(20μg) using MECP2 Antibody (HY-P84372) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MECP2 Antibody (YA4069)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 1(40μg) ) using MECP2 antibody(HY-P84372). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10
  • Experimental Validation Results for MECP2 Antibody (YA4069)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling MECP2 Antibody(HY-P84372
  • Experimental Validation Results for MECP2 Antibody (YA4069)
  • Experimental Validation Results for MECP2 Antibody (YA4069)
  • Experimental Validation Results for MECP2 Antibody (YA4069)
  • MECP2 Antibody (YA4069) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), A549 (lane 4(20μg) and A431 (lane 5(20μg) using MECP2 Antibody (HY-P84372) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MECP2 Antibody (YA4069) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431(lane 1(40μg) ) using MECP2 antibody(HY-P84372). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10

  • MECP2 Antibody (YA4069) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling MECP2 Antibody(HY-P84372

  • MECP2 Antibody (YA4069) Product Image WB
  • MECP2 Antibody (YA4069) Product Image WB
  • MECP2 Antibody (YA4069) Product Image FC