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CDKN2A/p16INK4a Antibody (YA508)

(Synonyms: CDK4 inhibitor p16 INK4 antibody; CDK4I antibody; CDKN2 antibody; CDKN2A antibody; Cell cycle negative regulator beta antibody; CMM2 antibody; Cyclin dependent kinase 4 inhibitor A antibody; Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody; Cyclin Dependent Kinase Inhibitor 2A antibody; Cyclin dependent kinase inhibitor 2A isoform 4 antibody; Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody; Cyclin dependent kinase inhibitor p16 antibody; INK4 antibody; INK4A antibody; MLM antibody; MTS1 antibody; Multiple tumor suppressor 1 antibody; p14 antibody; p16 antibody; P16INK4 antibody; p16INK4a antibody; p19 antibody; p19Arf antibody; TP16 antibody; )
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Based on 1 publication(s) in Google Scholar

CDKN2A/p16INK4a Antibody (YA508) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA508)
  • CDKN2A/p16INK4a Antibody (YA508) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

CDKN2A/p16INK4a Antibody (YA508) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human CDKN2A/p16INK4a p16 full length.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102633

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA508)
    Western blot analysis of extracts from NIH3T3(lane 2(20μg) and NIH3T3(lane 3(40μg) using CDKN2A/p16INK4a (HY-P80615) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:It is widely expressed, but not detected in the brain or skeletal muscle. Isomer 3 is pancreas-specific.

  • Isoforms & Post-Translational Modification

    P42771 has 6 isomers: P42771-1: 16533 Da (predicted); P42771-2: 11215 Da (predicted); P42771-3: 12083 Da (predicted); Q8N726-1: 13903 Da (predicted); P42771-4: 17883 Da (predicted); Q8N726-2: 8731 Da (predicted).
    Phosphorylation seems to increase interaction with CDK4

  • Subunit

    Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2

  • SwissProt ID

    P42771

  • Gene ID
    1029 [NCBI]
  • Synonyms

    CDK4 inhibitor p16 INK4 antibody; CDK4I antibody; CDKN2 antibody; CDKN2A antibody; Cell cycle negative regulator beta antibody; CMM2 antibody; Cyclin dependent kinase 4 inhibitor A antibody; Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody; Cyclin Dependent Kinase Inhibitor 2A antibody; Cyclin dependent kinase inhibitor 2A isoform 4 antibody; Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody; Cyclin dependent kinase inhibitor p16 antibody; INK4 antibody; INK4A antibody; MLM antibody; MTS1 antibody; Multiple tumor suppressor 1 antibody; p14 antibody; p16 antibody; P16INK4 antibody; p16INK4a antibody; p19 antibody; p19Arf antibody; TP16 antibody;

  • Research Field

    Cell Biology

CDKN2A/p16INK4a Antibody (YA508) Related Classifications

100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    CDKN2A/p16INK4a Antibody (YA508) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from NIH3T3(lane 2(20μg) and NIH3T3(lane 3(40μg) using CDKN2A/p16INK4a (HY-P80615) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CDKN2A/p16INK4a Antibody (YA508) Product Image
    1/16
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA508)
  • CDKN2A/p16INK4a Antibody (YA508) Product Image
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA508)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH3T3(lane 2(20μg) and NIH3T3(lane 3(40μg) using CDKN2A/p16INK4a (HY-P80615) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA508)
  • CDKN2A/p16INK4a Antibody (YA508) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH3T3(lane 2(20μg) and NIH3T3(lane 3(40μg) using CDKN2A/p16INK4a (HY-P80615) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CDKN2A/p16INK4a Antibody (YA508) Product Image WB