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ERp29 Antibody (YA1793)

(Synonyms: ERp28; ERP29_HUMAN; ERp31; HEL S 107; PDI-DB; PDIA9)
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Based on 1 Customer Validation

ERp29 Antibody (YA1793) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERp29.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ERp29 Antibody (YA1793)
  • ERp29 Antibody (YA1793) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200

Product Details

Description

ERp29 Antibody (YA1793) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERp29.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 29 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human ERp29 aa51-100/261.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ERp29 Antibody (YA1793)
    Western blot analysis of extracts from HEK293 (lane2, 20μg) and Hela (lane3, 20μg) using ERp29 Antibody (HY-P82048). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ERp29 is a Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER

  • Subcellular Localization

    Endoplasmic reticulum lumen; Melanosome

  • Expression


    Tissue_specificity:Ubiquitous. Mostly expressed in secretory tissues

  • Isoforms & Post-Translational Modification

    P30040 has 2 isomers: P30040-1: 28993 Da (predicted); P30040-2: 5477 Da (predicted).

  • Subunit

    Homodimer. Part of a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGGT1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX (PubMed:12475965)

  • SwissProt ID

    P30040

  • Gene ID
    10961 [NCBI]
  • Synonyms

    ERp28; ERP29_HUMAN; ERp31; HEL S 107; PDI-DB; PDIA9

  • Research Field

    Signal Transduction

ERp29 Antibody (YA1793) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane2, 20μg) and Hela (lane3, 20μg) using ERp29 Antibody (HY-P82048). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • ERp29 Antibody (YA1793) Product Image
    1/16
  • Experimental Validation Results for ERp29 Antibody (YA1793)
  • ERp29 Antibody (YA1793) Product Image
  • Experimental Validation Results for ERp29 Antibody (YA1793)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane2, 20μg) and Hela (lane3, 20μg) using ERp29 Antibody (HY-P82048). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ERp29 Antibody (YA1793)
  • ERp29 Antibody (YA1793) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane2, 20μg) and Hela (lane3, 20μg) using ERp29 Antibody (HY-P82048). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • ERp29 Antibody (YA1793) Product Image WB