MICA Antibody (YA1464)

Art. -Nr.: HY-P81719: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

MICA Antibody (YA1464) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MICA.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe	Verfügbarkeit	Menge
10 μL	Auf Lager	Estimated Time of Arrival: December 31
50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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MICA Antibody (YA1464) Spezialitätsempfehlungen:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung

Beschreibung

MICA Antibody (YA1464) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MICA.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molekulargewicht

Predicted band size: 43 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q29983

Gene ID

100507436 [NCBI]

Immunogen

Recombinant protein within human MICA aa 1-328.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000
FC FC: Flow Cytometry	1:5000
IP IP: Immunoprecipitation	1-2μg/sample

Sensitivity	Endogenous	Reinheit	affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa(lane 2(20μg), MCF-7(lane 3(20μg), A431(lane 4(20μg) and K562(lane 5(20μg) using MICA Antibody (HY-P81719). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

Function：Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed:11491531, PubMed:11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed:10426993)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cytoplasm

Expression：
Tissue_specificity:Besides the central nervous system, MICA is widely expressed in other tissues. It is primarily expressed in the gastric epithelium, but also in monocytes, keratinocytes, endothelial cells, fibroblasts, and the outer layer of normal thymic medullary Hardy bodies. In the skin, it is mainly expressed in the stratum corneum, basal cells, ducts, and follicles. MICA is also expressed in many (but not all) epithelial tumors of the lung, breast, kidney, ovary, prostate, and colon. In thymomas, MICA is overexpressed in both cortical and medullary epithelial cells. Tumors expressing MICA show elevated levels of γδT cells.

Induction:By heat shock, by infection with human cytomegalovirus (HCMV) , human adenovirus 5, M.tuberculosis and diarrheagenic E.coli, and by exposure to DNA damaging conditions such as high doses of ionizing radiation, chromatin-modifying treatments and inhibitors of DNA replication. The HCMV UL142 protein causes down-regulation of the full-length protein but not of the truncated MICA*008 allele

Isoforms & Post-Translational Modification：Q29983 has 2 isomers: Q29983-1: 42915 Da (predicted); Q29983-2: 31577 Da (predicted).
N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation;Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation;Palmitoylated on cysteine residues in the cytoplasmic tail leading to its association with membrane microdomains enriched in cholesterol;N-glycosylation is necessary for cell surface expression;(Microbial infection) Ubiquitinated by human herpesvirus 8 protein K5, leading to degradation

Subunit：Unlike classical MHC class I molecules, does not form a heterodimer with beta-2-microglobulin. Binds as a monomer to a KLRK1/NKG2D homodimer. KLRK1 forms a complex with HCST/DAP10 in which KLRK1 binds MICA while HCST acts as an adapter molecule which enables signal transduction. Interacts with PDIA6 on the surface of tumor cells, leading to disulfide bond reduction which is required for release of MICA from tumor cells

Database

Entrez Gene: 100507436 Human

SwissProt: Q29983 Human

OMIM: 177900 Human

Research Field

Immunology

Synonyms

PERB11.1; MICA; HLA class I antigen antibody; FLJ36918 antibody; FLJ60820 antibody; MGC111087 antibody; MGC21250 antibody; MHC class I chain related gene A protein antibody

Dokumentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

MICA Antibody (YA1464) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MICA Antibody (YA1464)

MICA Antibody (YA1464) Spezialitätsempfehlungen:

Product Detail

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Background

Beschreibung

MICA Antibody (YA1464) Related Classifications

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