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MICA Antibody (YA1464)

(Synonyms: PERB11.1; MICA; HLA class I antigen antibody; FLJ36918 antibody; FLJ60820 antibody; MGC111087 antibody; MGC21250 antibody; MHC class I chain related gene A protein antibody)
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Based on 1 Customer Validation

MICA Antibody (YA1464) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MICA.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MICA Antibody (YA1464)
  • Experimental Validation Results for MICA Antibody (YA1464)
  • MICA Antibody (YA1464) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000 1:5000 1-2μg/sample

Product Details

Description

MICA Antibody (YA1464) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MICA.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database
Immunogen

Recombinant protein within human MICA aa 1-328.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MICA Antibody (YA1464)
    Western blot analysis of extracts from HeLa(lane 2(20μg), MCF-7(lane 3(20μg), A431(lane 4(20μg) and K562(lane 5(20μg) using MICA Antibody (HY-P81719). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MICA Antibody (YA1464)
    Flow cytometric analysis of 1X106 HeLa cells labeling MICA Antibody (HY-P81719, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/5000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    MICA is widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production[1][2][3].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cytoplasm

  • Expression


    Tissue_specificity:Besides the central nervous system, MICA is widely expressed in other tissues. It is primarily expressed in the gastric epithelium, but also in monocytes, keratinocytes, endothelial cells, fibroblasts, and the outer layer of normal thymic medullary Hardy bodies. In the skin, it is mainly expressed in the stratum corneum, basal cells, ducts, and follicles. MICA is also expressed in many (but not all) epithelial tumors of the lung, breast, kidney, ovary, prostate, and colon. In thymomas, MICA is overexpressed in both cortical and medullary epithelial cells. Tumors expressing MICA show elevated levels of γδT cells.

    Induction:By heat shock, by infection with human cytomegalovirus (HCMV) , human adenovirus 5, M.tuberculosis and diarrheagenic E.coli, and by exposure to DNA damaging conditions such as high doses of ionizing radiation, chromatin-modifying treatments and inhibitors of DNA replication. The HCMV UL142 protein causes down-regulation of the full-length protein but not of the truncated MICA*008 allele

  • Isoforms & Post-Translational Modification

    Q29983 has 2 isomers: Q29983-1: 42915 Da (predicted); Q29983-2: 31577 Da (predicted).
    N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation;Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation;Palmitoylated on cysteine residues in the cytoplasmic tail leading to its association with membrane microdomains enriched in cholesterol;N-glycosylation is necessary for cell surface expression;(Microbial infection) Ubiquitinated by human herpesvirus 8 protein K5, leading to degradation

  • Subunit

    Unlike classical MHC class I molecules, does not form a heterodimer with beta-2-microglobulin. Binds as a monomer to a KLRK1/NKG2D homodimer. KLRK1 forms a complex with HCST/DAP10 in which KLRK1 binds MICA while HCST acts as an adapter molecule which enables signal transduction. Interacts with PDIA6 on the surface of tumor cells, leading to disulfide bond reduction which is required for release of MICA from tumor cells

  • SwissProt ID

    Q29983

  • Gene ID
    100507436 [NCBI]
  • Synonyms

    PERB11.1; MICA; HLA class I antigen antibody; FLJ36918 antibody; FLJ60820 antibody; MGC111087 antibody; MGC21250 antibody; MHC class I chain related gene A protein antibody

  • Research Field

    Immunology

MICA Antibody (YA1464) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg), MCF-7(lane 3(20μg), A431(lane 4(20μg) and K562(lane 5(20μg) using MICA Antibody (HY-P81719). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling MICA Antibody (HY-P81719, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/5000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MICA Antibody (YA1464) Product Image
    1/16
  • Experimental Validation Results for MICA Antibody (YA1464)
  • Experimental Validation Results for MICA Antibody (YA1464)
  • MICA Antibody (YA1464) Product Image
  • Experimental Validation Results for MICA Antibody (YA1464)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg), MCF-7(lane 3(20μg), A431(lane 4(20μg) and K562(lane 5(20μg) using MICA Antibody (HY-P81719). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MICA Antibody (YA1464)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling MICA Antibody (HY-P81719, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/5000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MICA Antibody (YA1464)
  • Experimental Validation Results for MICA Antibody (YA1464)
  • MICA Antibody (YA1464) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa(lane 2(20μg), MCF-7(lane 3(20μg), A431(lane 4(20μg) and K562(lane 5(20μg) using MICA Antibody (HY-P81719). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • MICA Antibody (YA1464) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling MICA Antibody (HY-P81719, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/5000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MICA Antibody (YA1464) Product Image WB
  • MICA Antibody (YA1464) Product Image FC