BRD3 Antibody
(Synonyms: KIAA0043, RING3L, BRD3, Bromodomain-containing protein 3, RING3-like protein)BRD3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to BRD3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-500 |
Product Details
BRD3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to BRD3.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 110; 75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 79 kDa
Synthetic peptide corresponding to the C-term region of human BRD3.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
BRD3 is a Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures. Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation. In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4. Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression. Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity)[1][2][3][4][5].
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Subcellular Localization
Nucleus; Chromosome
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Expression
Tissue_Specificity: Ubiquitous. -
Isoforms & Post-Translational Modification
BRD3 has 2 isoforms, Q15059-1: amino acid length is 726, molecular weight is 79542 Da (predicted); Q15059-2: amino acid length is 556, molecular weight is 60942 Da (predicted).
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Subunit
Interacts (via bromo domain 1) with GATA1 acetylated at 'Lys-312' and 'Lys-315' (By similarity).
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SwissProt ID
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Synonyms
KIAA0043, RING3L, BRD3, Bromodomain-containing protein 3, RING3-like protein
Documentation
[1]. LeRoy G, et al. The double bromodomain proteins Brd2 and Brd3 couple histone acetylation to transcription. Mol Cell. 2008 Apr 11;30(1):51-60. [Content Brief]
[2]. Filippakopoulos P, et al. Histone recognition and large-scale structural analysis of the human bromodomain family. Cell. 2012 Mar 30;149(1):214-31. [Content Brief]
[3]. Li Y, et al. Molecular Coupling of Histone Crotonylation and Active Transcription by AF9 YEATS Domain. Mol Cell. 2016 Apr 21;62(2):181-193. [Content Brief]
[4]. Daneshvar K, et al. lncRNA DIGIT and BRD3 protein form phase-separated condensates to regulate endoderm differentiation. Nat Cell Biol. 2020 Oct;22(10):1211-1222. [Content Brief]
[5]. Wai DCC, et al. The BRD3 ET domain recognizes a short peptide motif through a mechanism that is conserved across chromatin remodelers and transcriptional regulators. J Biol Chem. 2018 May 11;293(19):7160-7175. [Content Brief]
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