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KLC3 Antibody (YA10028)

(Synonyms: KLC2, KLC2L, KLC3, Kinesin light chain 3, KLC2-like, kinesin light chain 2)

KLC3 Antibody (YA10028) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to KLC3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KLC3 Antibody (YA10028)
  • KLC3 Antibody (YA10028) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-2000 1:100-200 1:50-200 1:10-50 1:10-50

Product Details

Description

KLC3 Antibody (YA10028) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to KLC3.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Immunogen

Synthetic peptide corresponding to human KLC3 protein.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KLC3 Antibody (YA10028)
    Western blot analysis was performed on protein extracts (25 μg) from Mouse testis (lane 2), U87 (lane 3), HeLa (lane 4), MCF-7 (lane 5), SH-SY5Y (lane 6), and HT-1080 (lane 7) using KLC3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    KLC3 is a Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Plays a role during spermiogenesis in the development of the sperm tail midpiece and in the normal function of spermatozoa (By similarity). May play a role in the formation of the mitochondrial sheath formation in the developing spermatid midpiece (By similarity)

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Mitochondrion

  • Isoforms & Post-Translational Modification

    KLC3 has 3 isoforms, Q6P597-1: amino acid length is 504, molecular weight is 55364 Da (predicted); Q6P597-2: amino acid length is 503, molecular weight is 55236 Da (predicted); Q6P597-3: amino acid length is 518, molecular weight is 56793 Da (predicted).

  • Subunit

    Oligomer composed of two heavy chains and two light chains.

  • SwissProt ID

    Q6P597

  • Gene ID
    147700 [NCBI]
  • Synonyms

    KLC2, KLC2L, KLC3, Kinesin light chain 3, KLC2-like, kinesin light chain 2

KLC3 Antibody (YA10028) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse testis (lane 2), U87 (lane 3), HeLa (lane 4), MCF-7 (lane 5), SH-SY5Y (lane 6), and HT-1080 (lane 7) using KLC3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • KLC3 Antibody (YA10028) Product Image
    1/16
  • Experimental Validation Results for KLC3 Antibody (YA10028)
  • KLC3 Antibody (YA10028) Product Image
  • Experimental Validation Results for KLC3 Antibody (YA10028)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse testis (lane 2), U87 (lane 3), HeLa (lane 4), MCF-7 (lane 5), SH-SY5Y (lane 6), and HT-1080 (lane 7) using KLC3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for KLC3 Antibody (YA10028)
  • KLC3 Antibody (YA10028) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Mouse testis (lane 2), U87 (lane 3), HeLa (lane 4), MCF-7 (lane 5), SH-SY5Y (lane 6), and HT-1080 (lane 7) using KLC3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • KLC3 Antibody (YA10028) Product Image WB