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T7 Tag Antibody (YA886)

Customer Review

Based on 1 Customer Validation

T7 Tag Antibody (YA886) is an unconjugated, mouse-derived, anti-T7 Tag (YA886) IgG1 monoclonal antibody.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for T7 Tag Antibody (YA886)
  • T7 Tag Antibody (YA886) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:10000

Product Details

Description

T7 Tag Antibody (YA886) is an unconjugated, mouse-derived, anti-T7 Tag (YA886) IgG1 monoclonal antibody.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Immunogen

Synthetic Peptide of T7-Tag

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3103168

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for T7 Tag Antibody (YA886)
    Western blot analysis was performed on extracts from Recombinant T7-Tag protein-10ng (lane 1, 10 ng), Recombinant T7-Tag protein-5ng (lane 2, 5 ng), Recombinant T7-Tag protein-2ng (lane 3, 2 ng), Recombinant T7-Tag protein-1ng (lane 4, 1 ng), Recombinant T7-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant T7-Tag protein-0.1ng (lane 6, 0.1 ng) using T7 Tag Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    T7 Tag is a T7 Tag Peptide is a protein tag consisting of 11 residues from the N-terminus of the T7 bacteriophage capsid protein gp10. T7 Tag Peptide can be fused to the N-terminus or C-terminus of proteins such as enhanced green fluorescent protein (EGFP). T7 Tag Peptide can be used in various immunoassays and affinity purification.

  • Research Field

    Tags & Cell Markers

T7 Tag Antibody (YA886) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Recombinant T7-Tag protein-10ng (lane 1, 10 ng), Recombinant T7-Tag protein-5ng (lane 2, 5 ng), Recombinant T7-Tag protein-2ng (lane 3, 2 ng), Recombinant T7-Tag protein-1ng (lane 4, 1 ng), Recombinant T7-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant T7-Tag protein-0.1ng (lane 6, 0.1 ng) using T7 Tag Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • T7 Tag Antibody (YA886) Product Image
    1/16
  • Experimental Validation Results for T7 Tag Antibody (YA886)
  • T7 Tag Antibody (YA886) Product Image
  • Experimental Validation Results for T7 Tag Antibody (YA886)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Recombinant T7-Tag protein-10ng (lane 1, 10 ng), Recombinant T7-Tag protein-5ng (lane 2, 5 ng), Recombinant T7-Tag protein-2ng (lane 3, 2 ng), Recombinant T7-Tag protein-1ng (lane 4, 1 ng), Recombinant T7-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant T7-Tag protein-0.1ng (lane 6, 0.1 ng) using T7 Tag Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for T7 Tag Antibody (YA886)
  • T7 Tag Antibody (YA886) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Recombinant T7-Tag protein-10ng (lane 1, 10 ng), Recombinant T7-Tag protein-5ng (lane 2, 5 ng), Recombinant T7-Tag protein-2ng (lane 3, 2 ng), Recombinant T7-Tag protein-1ng (lane 4, 1 ng), Recombinant T7-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant T7-Tag protein-0.1ng (lane 6, 0.1 ng) using T7 Tag Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • T7 Tag Antibody (YA886) Product Image WB