T7 Tag Antibody (YA886)

製品番号: HY-P81040: データシート SDS COA User Guide for Antibodies
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T7 Tag Antibody (YA886) is an unconjugated, mouse-derived, anti-T7 Tag (YA886) IgG1 monoclonal antibody.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$72	在庫あり	Estimated Time of Arrival: December 31
50 μL	$174	在庫あり	Estimated Time of Arrival: December 31
100 μL	$276	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

T7 Tag Antibody (YA886) is an unconjugated, mouse-derived, anti-T7 Tag (YA886) IgG1 monoclonal antibody.

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 55 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Species independent

Immunogen

Synthetic Peptide of T7-Tag

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:10000

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Recombinant T7-Tag protein-10ng (lane 1, 10 ng), Recombinant T7-Tag protein-5ng (lane 2, 5 ng), Recombinant T7-Tag protein-2ng (lane 3, 2 ng), Recombinant T7-Tag protein-1ng (lane 4, 1 ng), Recombinant T7-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant T7-Tag protein-0.1ng (lane 6, 0.1 ng) using T7 Tag Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：T7 Tag Peptide is a protein tag consisting of 11 residues from the N-terminus of the T7 bacteriophage capsid protein gp10. T7 Tag Peptide can be fused to the N-terminus or C-terminus of proteins such as enhanced green fluorescent protein (EGFP). T7 Tag Peptide can be used in various immunoassays and affinity purification.

RRID

AB_3103168

Research Field

Tags & Cell Markers

ドキュメンテーション

Select Batch:

データシート (230 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)