DOCK8 Antibody (YA9782)
(Synonyms: MRD2, ZIR8, HEL-205, Dedicator of cytokinesis protein 8)DOCK8 Antibody (YA9782) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to DOCK8.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, FC
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:200-500 | 1:10-100 |
Product Details
DOCK8 Antibody (YA9782) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to DOCK8.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 239 kDa;
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 239 kDa
Recombinant protein of human DOCK8
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
DOCK8 is a Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP. During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane. Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing[1][2][3].
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Subcellular Localization
Cytoplasm; Cell membrane; Cell projection, lamellipodium membrane
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Expression
Tissue_Specificity: Expressed in peripheral blood mononuclear cells (PBMCs). -
Isoforms & Post-Translational Modification
DOCK8 has 4 isoforms, Q8NF50-1: amino acid length is 2099, molecular weight is 238529 Da (predicted); Q8NF50-2: amino acid length is 2067, molecular weight is 235185 Da (predicted); Q8NF50-3: amino acid length is 2031, molecular weight is 230820 Da (predicted); Q8NF50-4: amino acid length is 1999, molecular weight is 227477 Da (predicted).In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1
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Subunit
Interacts (via DOCKER domain) with GTPase CDC42; the interaction activates CDC42 by exchanging GDP for GTP.
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SwissProt ID
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Synonyms
MRD2, ZIR8, HEL-205, Dedicator of cytokinesis protein 8
Documentation
[1]. Harada Y, et al. DOCK8 is a Cdc42 activator critical for interstitial dendritic cell migration during immune responses. Blood. 2012 May 10;119(19):4451-61. [Content Brief]
[2]. Xu X, et al. LRCH1 interferes with DOCK8-Cdc42-induced T cell migration and ameliorates experimental autoimmune encephalomyelitis. J Exp Med. 2017 Jan;214(1):209-226. [Content Brief]
[3]. Ham H, et al. HkRP3 is a microtubule-binding protein regulating lytic granule clustering and NK cell killing. J Immunol. 2015 Apr 15;194(8):3984-96. [Content Brief]
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