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Syntaxin 1B Antibody (YA1840)

(Synonyms: EPIM; Epimorphin; EPM; HPC 1; P35 1; STX1A; Stx1b; STX1B1; STX1B2; STX2; STX2A; STX2B; STX2C; STX3; STX3A)
Customer Review

Based on 1 Customer Validation

Syntaxin 1B Antibody (YA1840) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syntaxin 1B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Syntaxin 1B Antibody (YA1840)
  • Syntaxin 1B Antibody (YA1840) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50

Product Details

Description

Syntaxin 1B Antibody (YA1840) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syntaxin 1B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Syntaxin aa54-99.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104211

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Syntaxin 1B Antibody (YA1840)
    Western blot analysis of extracts from Rat brain tissue (lane2, 20μg), Rat brain tissue (lane3, 40μg), Mouse brain tissue (lane4, 20μg) and Mouse brain tissue (lane5, 40μg) using Syntaxin 1B Antibody (HY-P82095). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/750) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Syntaxin 1B is a Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm

  • Subcellular Localization

    Membrane; Single-pass type IV membrane protein; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle

  • Isoforms & Post-Translational Modification

    P61266 has 2 isomers: P61266-1: 33245 Da (predicted); P61266-2: 31787 Da (predicted).
    Phosphorylated by CK2

  • Subunit

    Interacts with OTOF. Interacts with SYT6 and SYT8; the interaction is Ca(2+)-dependent (By similarity)

  • SwissProt ID

    P61266

  • Gene ID
    112755 [NCBI]
  • Synonyms

    EPIM; Epimorphin; EPM; HPC 1; P35 1; STX1A; Stx1b; STX1B1; STX1B2; STX2; STX2A; STX2B; STX2C; STX3; STX3A

  • Research Field

    Neuroscience

Syntaxin 1B Antibody (YA1840) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Rat brain tissue (lane2, 20μg), Rat brain tissue (lane3, 40μg), Mouse brain tissue (lane4, 20μg) and Mouse brain tissue (lane5, 40μg) using Syntaxin 1B Antibody (HY-P82095). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/750) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Syntaxin 1B Antibody (YA1840) Product Image
    1/16
  • Experimental Validation Results for Syntaxin 1B Antibody (YA1840)
  • Syntaxin 1B Antibody (YA1840) Product Image
  • Experimental Validation Results for Syntaxin 1B Antibody (YA1840)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Rat brain tissue (lane2, 20μg), Rat brain tissue (lane3, 40μg), Mouse brain tissue (lane4, 20μg) and Mouse brain tissue (lane5, 40μg) using Syntaxin 1B Antibody (HY-P82095). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/750) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Syntaxin 1B Antibody (YA1840)
  • Syntaxin 1B Antibody (YA1840) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat brain tissue (lane2, 20μg), Rat brain tissue (lane3, 40μg), Mouse brain tissue (lane4, 20μg) and Mouse brain tissue (lane5, 40μg) using Syntaxin 1B Antibody (HY-P82095). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/750) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Syntaxin 1B Antibody (YA1840) Product Image WB