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Digoxigenin Antibody(YA3475)

(Synonyms: 1672-46-4 antibody; BRN 0096479 antibody; DIG antibody; EINECS 216-806-2 antibody; HSDB 7108 antibody; Lanadigenin antibody; Lanadigigenin antibody; ST056392 antibody; Digoxigenin)
Customer Review

Based on 1 Customer Validation

Digoxigenin Antibody(YA3475) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Digoxigenin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, IP, ELISA, Dot Blot, IF-Tissue

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Digoxigenin Antibody(YA3475)
  • Digoxigenin Antibody(YA3475) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
Dot Blot Info
 
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1:200-1:5000 1:5000-1:20000 1:50-1:1000 1:2000 1:50-1:1000 1-2 μg/sample

Product Details

Description

Digoxigenin Antibody(YA3475) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Digoxigenin.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Species independent
Immunogen

DIG-OVA

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718743

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Digoxigenin Antibody(YA3475)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Digoxigenin Antibody (HY-P83748A, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Digoxigenin is a Digoxigenin (HY-B1025)：Digoxigenin (DIG) is a steroid. DIG is used for situ hybridization as a labeling molecule probe due to long shelf life and fast detection and high sensitivity of DIG-labeled riboprobes.

  • Synonyms

    1672-46-4 antibody; BRN 0096479 antibody; DIG antibody; EINECS 216-806-2 antibody; HSDB 7108 antibody; Lanadigenin antibody; Lanadigigenin antibody; ST056392 antibody; Digoxigenin

Digoxigenin Antibody(YA3475) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Digoxigenin Antibody (HY-P83748A, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Digoxigenin Antibody(YA3475) Product Image
    1/16
  • Experimental Validation Results for Digoxigenin Antibody(YA3475)
  • Digoxigenin Antibody(YA3475) Product Image
  • Experimental Validation Results for Digoxigenin Antibody(YA3475)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Digoxigenin Antibody (HY-P83748A, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Digoxigenin Antibody(YA3475)
  • Digoxigenin Antibody(YA3475) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Digoxigenin Antibody (HY-P83748A, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Digoxigenin Antibody(YA3475) Product Image IHC-P