CHD1L Antibody (YA2236)

Cat. No.: HY-P82491: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

CHD1L Antibody (YA2236) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CHD1L.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CHD1L Antibody (YA2236) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CHD1L Antibody (YA2236) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CHD1L.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 101 kDa;

Observed band size: 101 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q86WJ1

Gene ID

9557 [NCBI]

Immunogen

A synthesized peptide derived from human CHD1L aa700-800.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:40
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A549 (lane 2(20μg)) and HEK293T (lane 3(20μg)) using CHD1L Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：ATP-dependent chromatin remodeler that mediates chromatin-remodeling following DNA damage (PubMed:19661379, PubMed:29220652, PubMed:29220653, PubMed:33357431, PubMed:34210977, PubMed:34486521, PubMed:34874266). Recruited to DNA damage sites through interaction with poly-ADP-ribose: specifically recognizes and binds histones that are poly-ADP-ribosylated on serine residues in response to DNA damage (PubMed:19661379, PubMed:29220652, PubMed:29220653, PubMed:34486521, PubMed:34874266). Poly-ADP-ribose-binding activates the ATP-dependent chromatin remodeler activity, thereby regulating chromatin during DNA repair (PubMed:19661379, PubMed:29220652, PubMed:29220653, PubMed:34486521, PubMed:34874266). Catalyzes nucleosome sliding away from DNA breaks in an ATP-dependent manner (PubMed:19661379, PubMed:29220652, PubMed:29220653). Chromatin remodeling activity promotes PARP2 removal from chromatin (PubMed:33275888)

Subcellular Localization：Nucleus; Chromosome

Expression：
Tissue_specificity:Frequently overexpressed in hepatomacellular carcinomas

Isoforms & Post-Translational Modification：Q86WJ1 has 5 isomers: Q86WJ1-1: 101000 Da (predicted); Q86WJ1-2: 90255 Da (predicted); Q86WJ1-3: 77755 Da (predicted); Q86WJ1-4: 88419 Da (predicted); Q86WJ1-5: 69155 Da (predicted).

Subunit：Interacts with nucleosomes; interacts with the acidic patch of histones (PubMed:33357431, PubMed:34210977, PubMed:34486521). Interacts (via macro domain) with PARP1; interacts only when PARP1 is poly-ADP-ribosylated (PARylated) (PubMed:19661379, PubMed:29220653). Interacts with CIAO1 (PubMed:23891004)

RRID

AB_3104598

Database

Entrez Gene: 9557 Human ; 68058 Mouse ;

SwissProt: Q86WJ1 Human ; Q9CXF7 Mouse ;

OMIM: 613039 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

ALC1; chd1l; CHDL

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

CHD1L Antibody (YA2236) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CHD1L Antibody (YA2236)

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CHD1L Antibody (YA2236) Related Classifications

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