**Mono-Methyl Arginine (R*GG) Antibody (YA6663)**

Cat. No.: HY-P86970: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Mono-Methyl Arginine (R*GG) Antibody (YA6663) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mono-Methyl Arginine (R*GG).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Mono-Methyl Arginine (R*GG) Antibody (YA6663) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mono-Methyl Arginine (R*GG).

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Observed band size: Mutiple kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Species independent

SwissProt ID

Q63009

Gene ID

60421 [NCBI]

Immunogen

Synthetic mono-methyl arginine peptide library containing R*GG motifs.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Methylated	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP, MAP3K5/ASK1 and MICU1 (PubMed:12737817, PubMed:15837430, PubMed:18492485, PubMed:19405910, PubMed:22095282). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-3' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation (By similarity). May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway (By similarity). Methylates RBM15, promoting ubiquitination and degradation of RBM15 (By similarity). Methylates MRE11 and TP53BP1, promoting the DNA damage response (By similarity). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity (By similarity). Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (By similarity). Methylates MAP3K5/ASK1 at 'Arg-85' and 'Arg-87' which promotes association of MAP3K5 with thioredoxin and negatively regulates MAP3K5 association with TRAF2, inhibiting MAP3K5 stimulation and MAP3K5-induced activation of JNK (PubMed:22095282). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (By similarity). Plays a role in regulating alternative splicing in the heart (By similarity). Methylates NPRL2 at 'Arg-78' leading to inhibition of its GTPase activator activity and then the GATOR1 complex and consequently inducing timely mTORC1 activation under methionine-sufficient conditions (By similarity)

Subcellular Localization：Nucleus,Nucleus, nucleoplasm,Cytoplasm, cytosol,Cytoplasm,Lysosome membrane

Expression：
Tissue_Specificity: Ubiquitous

Subunit：Homodimer and heterodimer with PRMT8

Synonyms

Arginine (mono methyl) antibody; Mono-Methyl Arginine (R*GG)

Documentation

User Guide for Antibodies (1077 KB)