NCAPH Antibody
(Synonyms: BRRN, BRRN1, CAPH, KIAA0074, NCAPH, Condensin complex subunit 2, Barren homolog protein 1, Chromosome-associated protein H, Non-SMC condensin I complex subunit H, XCAP-H homolog, hCAP-H)NCAPH Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to NCAPH.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
NCAPH Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to NCAPH.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 97 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 82 kDa
Synthetic peptide corresponding to the center region of human NCAPH.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NCAPH is a Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size[1][2].
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Subcellular Localization
Nucleus; Cytoplasm; Chromosome
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Expression
Tissue_Specificity: Widely expressed at low level. Expressed in proliferating cells. -
Isoforms & Post-Translational Modification
NCAPH has 2 isoforms, Q15003-1: amino acid length is 741, molecular weight is 82563 Da (predicted); Q15003-2: amino acid length is 605, molecular weight is 67495 Da (predicted).可被 CDK1 磷酸化。该蛋白以及 NCAPD2、NCAPG 亚基的磷酸化会激活凝缩蛋白复合物，是染色体凝缩所必需的(根据相似性推断)NCAPH 存在 2 个异构体，Q15003-1：氨基酸个数为 741 个，分子量为 82563 Da (预测)；Q15003-2：氨基酸个数为 605 个，分子量为 67495 Da (预测)。Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation (By similarity)
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Subunit
Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: NCAPH/BRRN1, NCAPD2/CAPD2 and NCAPG
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SwissProt ID
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Synonyms
BRRN, BRRN1, CAPH, KIAA0074, NCAPH, Condensin complex subunit 2, Barren homolog protein 1, Chromosome-associated protein H, Non-SMC condensin I complex subunit H, XCAP-H homolog, hCAP-H
Documentation
[1]. Kimura K, et al. Chromosome condensation by a human condensin complex in Xenopus egg extracts. J Biol Chem. 2001 Feb 23;276(8):5417-20. [Content Brief]
[2]. Martin CA, et al. Mutations in genes encoding condensin complex proteins cause microcephaly through decatenation failure at mitosis. Genes Dev. 2016 Oct 1;30(19):2158-2172. [Content Brief]
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