TNMD Antibody
(Synonyms: BRICD4; BRICHOS domain containing 4; CHM1 LIKE; CHM1L; Chondromodulin I like; Chondromodulin I like protein; Chondromodulin-1-like protein; Chondromodulin-I-like protein; hChM1L; hTeM; Myodulin; TEM; Tendin; Tenomodulin; Tenomodulin protein; TNMD; TNMD_HUMAN; UNQ771/PRO1565.)Based on 1 Customer Validation
TNMD Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to TNMD.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
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| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:100-500 | 1:100-500 |
Product Details
TNMD Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to TNMD.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat Predicted Reactivity: Dog,Pig,Cow,Horse,Rabbit,SheepNote: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Observed Molecular WeightObserved band size: 35 kDaNote: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 37 kDa
Entrez Gene: 64102 Human ; 64103 Mouse ; 64104 Rat
SwissProt: Q9H2S6 Human ; Q9EP64 Mouse ; Q9ESC2 Rat
OMIM: 300459 Human
KLH conjugated synthetic peptide derived from human TNMD: 151-250/317
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Mouse Spleen(lane 2(20μg), Rat Heart(lane 3(20μg) and Mouse Heart(lane 4(20μg) using TNMD Antibody (HY-P81207). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993,1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TNMD Antibody (HY-P81207, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using TNMD Antibody (HY-P81207, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using TNMD Antibody (HY-P81207, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using TNMD Antibody (HY-P81207, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using TNMD Antibody (HY-P81207, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using TNMD Antibody (HY-P81207, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human endometrium cancer tissue using TNMD Antibody (HY-P81207, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using TNMD Antibody (HY-P81207, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
TNMD may be an angiogenesis inhibitor
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Subcellular Localization
Membrane; Single-pass type II membrane protein; Nucleus envelope; Membrane; Single-pass type II membrane protein; Nucleus envelope; Cytoplasm
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Expression
Tissue_specificity:Highly expressed in hypovascular connective tissues such as tendons. Also has strong expression in adipose tissue -
Isoforms & Post-Translational Modification
Q9H2S6 has 3 isomers: Q9H2S6-1: 37130 Da (predicted); Q9H2S6-2: 23967 Da (predicted); Q9H2S6-3: 30013 Da (predicted).
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SwissProt ID
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Synonyms
BRICD4; BRICHOS domain containing 4; CHM1 LIKE; CHM1L; Chondromodulin I like; Chondromodulin I like protein; Chondromodulin-1-like protein; Chondromodulin-I-like protein; hChM1L; hTeM; Myodulin; TEM; Tendin; Tenomodulin; Tenomodulin protein; TNMD; TNMD_HUMAN; UNQ771/PRO1565.
Documentation
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Data Sheet (231 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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User Guide for Antibodies (1077 KB)