SMN1 Antibody (YA4315)(PBS only)

Cat. No.: HY-P84618A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

SMN1 Antibody (YA4315) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SMN1.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

SMN1 Antibody (YA4315) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SMN1.

Host

Mouse

분자량

Predicted band size: 32 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Monkey

SwissProt ID

Q16637

Gene ID

6606 [NCBI]

Immunogen

Purified recombinant fragment of human SMN1 aa 148-294.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed:18984161, PubMed:9845364). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (PubMed:18984161). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed:18984161). To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate (PubMed:18984161). Within the SMN complex, SMN1 acts as a structural backbone and together with GEMIN2 it gathers the Sm complex subunits (PubMed:17178713, PubMed:21816274, PubMed:22101937). Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP (PubMed:31799625). Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development (PubMed:23063131). Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination (PubMed:26700805). May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs)

Subcellular Localization：Nucleus, gem; Nucleus, Cajal body; Cytoplasm; Cytoplasmic granule; Perikaryon; Cell projection, neuron projection; Cell projection, axon; Cytoplasm, myofibril, sarcomere, Z line

Expression：
Tissue_specificity:It is expressed in a variety of tissues. High expression is observed in the brain, kidneys, and liver; moderate expression in skeletal muscle and cardiac muscle; and low expression in fibroblasts and lymphocytes. High expression is also observed in the spinal cord. Expression is also found in osteoclasts and monocytes (protein level) .

Isoforms & Post-Translational Modification：Q16637 has 4 isomers: Q16637-1: 31849 Da (predicted); Q16637-2: 28534 Da (predicted); Q16637-3: 30450 Da (predicted); Q16637-4: 27135 Da (predicted).

Subunit：Homooligomer; may form higher order homooligomers in the dimer to octamer range (PubMed:10500148, PubMed:14715275, PubMed:17178713, PubMed:21816274, PubMed:23022347, PubMed:26092730). Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP (PubMed:17178713, PubMed:9323129). Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG (PubMed:12065586, PubMed:18984161). Component of an import snRNP complex composed of KPNB1, RNUT1, SMN1 and ZNF259 (PubMed:12095920). Interacts with DDX20, FBL, NOLA1, RNUT1, SYNCRIP and with several spliceosomal snRNP core Sm proteins, including SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE and ILF3 (PubMed:10500148, PubMed:11574476, PubMed:12095920). Interacts with GEMIN2; the interaction is direct (PubMed:10500148, PubMed:17178713, PubMed:21816274, PubMed:22607171, PubMed:23022347, PubMed:26092730, PubMed:31799625). Interacts with GEMIN3; the interaction is direct (PubMed:17178713). Interacts with GEMIN8; the interaction is direct (PubMed:17178713, PubMed:33754639). Interacts with SNRPB; the interaction is direct (PubMed:10500148). Interacts (via Tudor domain) with SNRPD1 (via C-terminus); the interaction is direct (PubMed:10500148, PubMed:11135666). Interacts with SNRPD2; the interaction is direct (PubMed:10500148). Interacts (via Tudor domain) with SNRPD3 (via C-terminus); the interaction is direct (PubMed:10500148, PubMed:11135666, PubMed:12628254, PubMed:22101937). Interacts with SNRPE; the interaction is direct (PubMed:10500148). Interacts with OSTF1, LSM10, LSM11 and RPP20/POP7 (PubMed:11551898, PubMed:12975319, PubMed:14715275, PubMed:16087681). Interacts (via C-terminal region) with ZPR1 (via C-terminal region) (PubMed:11283611). Interacts (via Tudor domain) with COIL (PubMed:11641277). Interacts with SETX; recruits SETX to POLR2A (PubMed:21700224, PubMed:26700805). Interacts with POLR2A (via the C-terminal domain (CTD)) (PubMed:26700805). Interacts with PRMT5 (PubMed:26700805). Interacts with XRN2 (PubMed:26700805). Interacts (via C-terminus) with FMR1 (via C-terminus); the interaction is direct and occurs in a RNA-independent manner (PubMed:18093976). Interacts (via Tudor domain) with SF3B2 ('Arg-508'-methylated form) (PubMed:25737013). Interacts with WRAP53/TCAB1 (PubMed:21072240). Interacts (via Tudor domain) with ELAVL4 in an RNA-independent manner; the interaction is required for localization of ELAVL4 to RNA granules (PubMed:21088113, PubMed:21389246, PubMed:29061699). Interacts with FRG1 (PubMed:17103222)

Synonyms

SMA; SMN; SMA1; SMA2; SMA3; SMA4; SMA@; SMN2; SMNT; BCD541; T-BCD541

각종 서류

User Guide for Antibodies (1077 KB)