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PPCS Antibody (YA3177)

(Synonyms: CMD2C)
Customer Review

Based on 1 Customer Validation

PPCS Antibody (YA3177) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PPCS.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PPCS Antibody (YA3177)
  • PPCS Antibody (YA3177) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

PPCS Antibody (YA3177) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PPCS.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 34 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Species Reactivity Database

Entrez Gene: 79717 Human

SwissProt: Q9HAB8 Human

Immunogen

A synthetic peptide of human PPCS

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105543

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PPCS Antibody (YA3177)
    Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), 293T (lane 2, 20 μg), HepG2 (lane 3, 20 μg), and Jurkat (lane 4, 20 μg) using PPCS Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    PPCS catalyzes the second step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. Has a preference for ATP over CTP as a cosubstrate[1][2][3].

  • Isoforms & Post-Translational Modification

    Q9HAB8 has 2 isomers: Q9HAB8-1: 34005 Da (predicted); Q9HAB8-2: 15645 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    Q9HAB8

  • Gene ID
    79717 [NCBI]
  • Synonyms

    CMD2C

  • Research Field

    Signal Transduction

PPCS Antibody (YA3177) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), 293T (lane 2, 20 μg), HepG2 (lane 3, 20 μg), and Jurkat (lane 4, 20 μg) using PPCS Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PPCS Antibody (YA3177) Product Image
    1/16
  • Experimental Validation Results for PPCS Antibody (YA3177)
  • PPCS Antibody (YA3177) Product Image
  • Experimental Validation Results for PPCS Antibody (YA3177)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), 293T (lane 2, 20 μg), HepG2 (lane 3, 20 μg), and Jurkat (lane 4, 20 μg) using PPCS Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PPCS Antibody (YA3177)
  • PPCS Antibody (YA3177) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), 293T (lane 2, 20 μg), HepG2 (lane 3, 20 μg), and Jurkat (lane 4, 20 μg) using PPCS Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PPCS Antibody (YA3177) Product Image WB