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PUS1 Antibody (YA1783)

(Synonyms: MLASA1)
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Based on 1 Customer Validation

PUS1 Antibody (YA1783) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PUS1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Hamster, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PUS1 Antibody (YA1783)
  • PUS1 Antibody (YA1783) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

PUS1 Antibody (YA1783) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PUS1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Hamster, Rat
  • Observed Molecular Weight
    Observed band size: 44,47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human PUS1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104159

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PUS1 Antibody (YA1783)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Daudi (lane 2, 15 μg), A431 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using PUS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    PUS1 is a Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions. Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA[1][2][3][4].

  • Subcellular Localization

    Mitochondrion; Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Widely expressed (PubMed:15108122) . High levels of expression found in brain and skeletal muscle (PubMed:15108122)

  • Isoforms & Post-Translational Modification

    Q9Y606 has 2 isomers: Q9Y606-1: 47470 Da (predicted); Q9Y606-2: 44377 Da (predicted).

  • Subunit

    Monomer (PubMed:23707380, PubMed:24722331). Forms a complex with RARG and the SRA1 RNA in the nucleus (By similarity)

  • SwissProt ID

    Q9Y606

  • Gene ID
    80324 [NCBI]
  • Synonyms

    MLASA1

  • Research Field

    Epigenetics and Nuclear Signaling

PUS1 Antibody (YA1783) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Daudi (lane 2, 15 μg), A431 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using PUS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PUS1 Antibody (YA1783) Product Image
    1/16
  • Experimental Validation Results for PUS1 Antibody (YA1783)
  • PUS1 Antibody (YA1783) Product Image
  • Experimental Validation Results for PUS1 Antibody (YA1783)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Daudi (lane 2, 15 μg), A431 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using PUS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PUS1 Antibody (YA1783)
  • PUS1 Antibody (YA1783) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Daudi (lane 2, 15 μg), A431 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using PUS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PUS1 Antibody (YA1783) Product Image WB