uPAR Antibody (YA5516)

Cat. No.: HY-P85824: Data Sheet User Guide for Antibodies
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uPAR Antibody (YA5516) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to uPAR.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

uPAR Antibody (YA5516) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to uPAR.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 37 kDa;

Observed band size: 37 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q03405

Gene ID

5329 [NCBI]

Immunogen

Synthesized peptide derived from human uPAR AA range: 100-200

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-5000

Purity	Protein G	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a receptor for urokinase plasminogen activator (PubMed:15677461). Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form

Subcellular Localization：Cell membrane; Cell projection, invadopodium membrane; Cell membrane; Lipid-anchor, GPI-anchor; Secreted

Expression：
Tissue_specificity:It is expressed (protein level) in neurons in the central region of the brain. It is expressed in the brain.

Isoforms & Post-Translational Modification：Q03405 has 3 isomers: Q03405-1: 36978 Da (predicted); Q03405-2: 31263 Da (predicted); Q03405-3: 32016 Da (predicted).

Subunit：Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Interacts with FAP (seprase); the interaction occurs at the cell surface of invadopodia membrane. Interacts with SORL1 (via N-terminal ectodomain); this interaction decreases PLAUR internalization (PubMed:14764453, PubMed:23486467). The ternary complex composed of PLAUR-PLAU-SERPINE1 also interacts with SORL1 (PubMed:15053742). Interacts with CD82; this interaction prevents PLAUR from binding to its high affinity ligand PLAU (PubMed:15677461)

Documentation

User Guide for Antibodies (1077 KB)