EXO1 Antibody
(Synonyms: EXOI, HEX1, EXO1, Exonuclease 1, hExo1, Exonuclease I, hExoI)EXO1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to EXO1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, ChIP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ChIP
ChIP: Chromatin Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-500 | 1:100-500 |
Product Details
EXO1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to EXO1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 110 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 94 kDa
Synthetic peptide corresponding to the N-term region of human EXO1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
EXO1 is a 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. -
Isoforms & Post-Translational Modification
EXO1 has 2 isoforms, Q9UQ84-1: amino acid length is 846, molecular weight is 94103 Da (predicted); Q9UQ84-4: amino acid length is 803, molecular weight is 89231 Da (predicted).
Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilization/degradation of the protein. -
Subunit
Interacts with the MLH1-PMS2 heterodimer via MLH1.
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SwissProt ID
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Synonyms
EXOI, HEX1, EXO1, Exonuclease 1, hExo1, Exonuclease I, hExoI
Documentation
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