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Glutathione S Transferase mu 1 Antibody (YA2308)

(Synonyms: MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; MGC26563; GSTM1a-1a; GSTM1b-1b)
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Based on 1 Customer Validation

Glutathione S Transferase mu 1 Antibody (YA2308) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutathione S Transferase mu 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glutathione S Transferase mu 1 Antibody (YA2308)
  • Glutathione S Transferase mu 1 Antibody (YA2308) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Glutathione S Transferase mu 1 Antibody (YA2308) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutathione S Transferase mu 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 26 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 26 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Glutathione S Transferase mu aa100-152/218.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104664

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glutathione S Transferase mu 1 Antibody (YA2308)
    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), Hela (lane 2, 20 μg), HepG2 (lane 3, 20 μg), U2OS (lane 4, 20 μg), and 3T3 (lane 5, 20 μg) using GSTM1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Glutathione S Transferase mu 1 is a Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). Participates in the formation of novel hepoxilin regioisomers[1][2].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Liver (protein levels)

  • Isoforms & Post-Translational Modification

    P09488 has 2 isomers: P09488-1: 25712 Da (predicted); P09488-2: 21253 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    P09488

  • Gene ID
    2944 [NCBI]
  • Synonyms

    MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; MGC26563; GSTM1a-1a; GSTM1b-1b

  • Research Field

    Signal Transduction

Glutathione S Transferase mu 1 Antibody (YA2308) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), Hela (lane 2, 20 μg), HepG2 (lane 3, 20 μg), U2OS (lane 4, 20 μg), and 3T3 (lane 5, 20 μg) using GSTM1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Glutathione S Transferase mu 1 Antibody (YA2308) Product Image
    1/16
  • Experimental Validation Results for Glutathione S Transferase mu 1 Antibody (YA2308)
  • Glutathione S Transferase mu 1 Antibody (YA2308) Product Image
  • Experimental Validation Results for Glutathione S Transferase mu 1 Antibody (YA2308)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), Hela (lane 2, 20 μg), HepG2 (lane 3, 20 μg), U2OS (lane 4, 20 μg), and 3T3 (lane 5, 20 μg) using GSTM1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Glutathione S Transferase mu 1 Antibody (YA2308)
  • Glutathione S Transferase mu 1 Antibody (YA2308) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), Hela (lane 2, 20 μg), HepG2 (lane 3, 20 μg), U2OS (lane 4, 20 μg), and 3T3 (lane 5, 20 μg) using GSTM1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Glutathione S Transferase mu 1 Antibody (YA2308) Product Image WB