Wnt-10b Antibody (YA3495)(PBS only)

Cat. No.: HY-P83798A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Wnt-10b Antibody (YA3495) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Wnt-10b.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Wnt-10b Antibody (YA3495) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Wnt-10b.

Host

Mouse

Molecular Weight

Predicted band size: 43 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O00744

Gene ID

7480 [NCBI]

Immunogen

Purified recombinant fragment of human WNT10B aa 85-389.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin

Subcellular Localization：Secreted, extracellular space, extracellular matrix; Secreted

Expression：
Tissue_specificity:It has been detected in most adult tissues. The highest concentrations are found in the heart and skeletal muscle. Lower concentrations are found in brain tissue.

Isoforms & Post-Translational Modification：O00744 has 2 isomers: O00744-1: 43000 Da (predicted); O00744-2: 20483 Da (predicted).
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition

Subunit：Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720)

Synonyms

SHFM6; WNT-12

Documentation

User Guide for Antibodies (1077 KB)