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14-3-3 beta Antibody (YA3955)

(Synonyms: HS1; GW128; YWHAA; KCIP-1; HEL-S-1)
Customer Review

Based on 1 Customer Validation

14-3-3 beta Antibody (YA3955) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to 14-3-3 beta.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • 14-3-3 beta Antibody (YA3955) Product Image
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Amount: USD 0.00
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

14-3-3 beta Antibody (YA3955) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to 14-3-3 beta.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Immunogen

Purified recombinant fragment of human YWHAB (AA: 1-246) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718868

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    Western blot analysis of extracts from HEK293T (lane 2(20μg), HEK293 (lane 3(20μg), Raji (lane 4(20μg), HepG2 (lane 5(20μg) using 14-3-3 beta Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    Immunohistochemical analysis of paraffin-embedded human cervix tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    Immunohistochemical analysis of paraffin-embedded human breast tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    Immunocytochemistry analysis of HeLa cells labeling 14-3-3 beta with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue）
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    Immunocytochemistry analysis of PC-3 cells labeling 14-3-3 beta with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13

  • Subcellular Localization

    Cytoplasm; Melanosome; Vacuole membrane

  • Isoforms & Post-Translational Modification

    14-3-3 beta has 2 isomers: P31946-1: 28082 Da (predicted); P31946-2: 27850 Da (predicted).
    The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion

  • Subunit

    Homodimer (PubMed:17717073). Interacts with SAMSN1 and PRKCE (By similarity). Interacts with AKAP13 (PubMed:21224381).

  • SwissProt ID

    P31946

  • Gene ID
    7529 [NCBI]
  • Synonyms

    HS1; GW128; YWHAA; KCIP-1; HEL-S-1

References

[1]. Obsilova V, et al. Structural insights into the functional roles of 14-3-3 proteins. Front Mol Biosci. 2022;9:1016071. [Content Brief]

[2]. Muslin AJ, et al. Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine. Cell. 1996;84(6):889-897. [Content Brief]

[3]. Yang X, et al. Structural basis for protein-protein interactions in the 14-3-3 protein family. Proc Natl Acad Sci U S A. 2006;103(46):17237-17242. [Content Brief]

[4]. Gardino AK, et al. 14-3-3 proteins as signaling integration points for cell cycle control and apoptosis. Semin Cell Dev Biol. 2011;22(7):688-695. [Content Brief]

[5]. Pennington KL, et al. The dynamic and stress-adaptive signaling hub of 14-3-3: emerging mechanisms of regulation and context-dependent protein-protein interactions. Oncogene. 2018;37(42):5587-5604. [Content Brief]

[6]. Li Z, et al. 14-3-3σ, the double-edged sword of human cancers. Am J Transl Res. 2009;1(4):326-340. [Content Brief]

[7]. Maksymowych WP, et al. Serum 14-3-3η is a novel marker that complements current serological measurements to enhance detection of patients with rheumatoid arthritis. J Rheumatol. 2014;41(11):2104-2113. [Content Brief]

[8]. Aghazadeh Y, et al. The role of the 14-3-3 protein family in health, disease, and drug development. Drug Discov Today. 2016;21(2):278-287. [Content Brief]

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14-3-3 beta Antibody (YA3955) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane 2(20μg), HEK293 (lane 3(20μg), Raji (lane 4(20μg), HepG2 (lane 5(20μg) using 14-3-3 beta Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling 14-3-3 beta with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue）

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling 14-3-3 beta with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 14-3-3 beta Antibody (YA3955) Product Image
    1/16
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • 14-3-3 beta Antibody (YA3955) Product Image
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane 2(20μg), HEK293 (lane 3(20μg), Raji (lane 4(20μg), HepG2 (lane 5(20μg) using 14-3-3 beta Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling 14-3-3 beta with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue）
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling 14-3-3 beta with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • Experimental Validation Results for 14-3-3 beta Antibody (YA3955)
  • 14-3-3 beta Antibody (YA3955) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T (lane 2(20μg), HEK293 (lane 3(20μg), Raji (lane 4(20μg), HepG2 (lane 5(20μg) using 14-3-3 beta Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 14-3-3 beta Antibody (YA3955) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 14-3-3 beta Antibody (YA3955) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 14-3-3 beta Antibody (YA3955) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 14-3-3 beta Antibody (YA3955) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 14-3-3 beta Antibody (YA3955) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 14-3-3 beta Antibody (YA3955) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using 14-3-3 beta Antibody (HY-P84258, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 14-3-3 beta Antibody (YA3955) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling 14-3-3 beta with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue）

  • 14-3-3 beta Antibody (YA3955) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling 14-3-3 beta with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with 14-3-3 beta Antibody (HY-P84258) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 14-3-3 beta Antibody (YA3955) Product Image WB
  • 14-3-3 beta Antibody (YA3955) Product Image IHC-P
  • 14-3-3 beta Antibody (YA3955) Product Image IHC-P
  • 14-3-3 beta Antibody (YA3955) Product Image IHC-P
  • 14-3-3 beta Antibody (YA3955) Product Image IHC-P
  • 14-3-3 beta Antibody (YA3955) Product Image IHC-P
  • 14-3-3 beta Antibody (YA3955) Product Image IHC-P
  • 14-3-3 beta Antibody (YA3955) Product Image ICC
  • 14-3-3 beta Antibody (YA3955) Product Image ICC