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MERTK Antibody (YA2966)

(Synonyms: MERTK; MER; Tyrosine-protein kinase Mer; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK)
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Based on 1 Customer Validation

MERTK Antibody (YA2966) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MERTK.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MERTK Antibody (YA2966)
  • MERTK Antibody (YA2966) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50

Product Details

Description

MERTK Antibody (YA2966) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MERTK.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 188 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 110 kDa
Species Reactivity Database

Entrez Gene: 10461 Human

SwissProt: Q12866 Human

Immunogen

A synthesized peptide derived from human MERTK aa25-48.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105335

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MERTK Antibody (YA2966)
    Western blot analysis of extracts from HEK293T(lane 2(20ug) , MCF-7(lane 3(20ug) and HEK293(lane 4(20ug) using MERTK Antibody (HY-P83221) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    MERTK receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3[1].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is not expressed in normal B cells and T lymphocytes, but is expressed in various neoplastic B cell and T cell lines. It is highly expressed in the testes, ovaries, prostate, lungs, and kidneys, and less expressed in the spleen, small intestine, colon, and liver.

  • Subunit

    Interacts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6 (By similarity). Interacts with VAV1 in a phosphotyrosine-independent manner. Interacts with TIMD4; this interaction enhances TIMD4-mediated efferocytosis (PubMed:32640697)

  • SwissProt ID

    Q12866

  • Gene ID
    10461 [NCBI]
  • Synonyms

    MERTK; MER; Tyrosine-protein kinase Mer; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK

  • Research Field

    Neuroscience

MERTK Antibody (YA2966) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) , MCF-7(lane 3(20ug) and HEK293(lane 4(20ug) using MERTK Antibody (HY-P83221) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MERTK Antibody (YA2966) Product Image
    1/16
  • Experimental Validation Results for MERTK Antibody (YA2966)
  • MERTK Antibody (YA2966) Product Image
  • Experimental Validation Results for MERTK Antibody (YA2966)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) , MCF-7(lane 3(20ug) and HEK293(lane 4(20ug) using MERTK Antibody (HY-P83221) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MERTK Antibody (YA2966)
  • MERTK Antibody (YA2966) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T(lane 2(20ug) , MCF-7(lane 3(20ug) and HEK293(lane 4(20ug) using MERTK Antibody (HY-P83221) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MERTK Antibody (YA2966) Product Image WB