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Transferrin Antibody (YA5374)

(Synonyms: TF; Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin)
Customer Review

Based on 1 Customer Validation

Transferrin Antibody (YA5374) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Transferrin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, IHC-P, ICC/IF,

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Transferrin Antibody (YA5374) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-2000 1:200 1:50-300

Product Details

Description

Transferrin Antibody (YA5374) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Transferrin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 77 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Synthetic Peptide of Transferrin

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Transferrin Antibody (YA5374)
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2) using Transferrin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    Western blot analysis of extracts from HepG2 (lane2, 40μg) and K562 (lane3, 40μg) using Transferrin Antibody (HY-P85682). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight.Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    Immunohistochemical analysis of paraffin-embedded human testis cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Transferrin is a Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation; (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use; (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6:ESAG7 and extract its iron for its own use

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:It is expressed by the liver and secreted into the blood plasma.

  • Subunit

    Monomer (Probable) (PubMed:22327295). Part of a complex composed of SLC40A1/ferroportin, TF/transferrin and HEPH/hephaestin that transfers iron from cells to transferrin (PubMed:37277838)

  • SwissProt ID

    P02787

  • Gene ID
    7018 [NCBI]
  • Synonyms

    TF; Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin

Transferrin Antibody (YA5374) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2) using Transferrin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2, 40μg) and K562 (lane3, 40μg) using Transferrin Antibody (HY-P85682). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight.Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Transferrin Antibody (YA5374) Product Image
    1/16
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Transferrin Antibody (YA5374) Product Image
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2) using Transferrin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2, 40μg) and K562 (lane3, 40μg) using Transferrin Antibody (HY-P85682). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight.Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Experimental Validation Results for Transferrin Antibody (YA5374)
  • Transferrin Antibody (YA5374) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2) using Transferrin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Transferrin Antibody (YA5374) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane2, 40μg) and K562 (lane3, 40μg) using Transferrin Antibody (HY-P85682). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight.Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • Transferrin Antibody (YA5374) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Transferrin Antibody (YA5374) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Transferrin Antibody (YA5374) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Transferrin Antibody (YA5374) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Transferrin Antibody (YA5374) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Transferrin Antibody (YA5374) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Transferrin Antibody (YA5374) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Transferrin Antibody (YA5374) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using Transferrin Antibody (HY-P85682, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Transferrin Antibody (YA5374) Product Image WB
  • Transferrin Antibody (YA5374) Product Image WB
  • Transferrin Antibody (YA5374) Product Image IHC-P
  • Transferrin Antibody (YA5374) Product Image IHC-P
  • Transferrin Antibody (YA5374) Product Image IHC-P
  • Transferrin Antibody (YA5374) Product Image IHC-P
  • Transferrin Antibody (YA5374) Product Image IHC-P
  • Transferrin Antibody (YA5374) Product Image IHC-P
  • Transferrin Antibody (YA5374) Product Image IHC-P
  • Transferrin Antibody (YA5374) Product Image IHC-P