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HuR Antibody (YA1933)

(Synonyms: HUR; Hua; MelG; ELAV1)
Customer Review

Based on 1 Customer Validation

HuR Antibody (YA1933) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HuR.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL IHC-P FC
  • Experimental Validation Results for HuR Antibody (YA1933)
  • Experimental Validation Results for HuR Antibody (YA1933)
  • Experimental Validation Results for HuR Antibody (YA1933)
  • HuR Antibody (YA1933) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:40 1:50-1:100

Product Details

Description

HuR Antibody (YA1933) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HuR.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human HuR / ELAVL1

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104301

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HuR Antibody (YA1933)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using HuR Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HuR Antibody (YA1933)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using HuR Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HuR Antibody (YA1933)
    Flow cytometric analysis of 1X10^6 Hela cells labeling HuR Antibody (YA1933) (HY-P82188, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Human Antigen R (HuR), also known as ELAV-like protein 1 (ELAVL1), is an RNA-binding protein that plays a crucial role in the process of RNA metabolism within cells. HuR enhances the stability of mRNA and guides the mRNA to be transported from the nucleus to the cytoplasm through the nuclear pore complex, ensuring that the mRNA reaches the ribosomes for translation. HuR can also interact with translation initiation factors to promote the binding of ribosomes to mRNA, thereby regulating the translation efficiency of mRNA and affecting protein synthesis. By regulating the stability, transportation, and translation of the mRNAs of related genes, HuR plays an important role in tumors, neurodegenerative diseases, and inflammatory diseases[1][2].

  • Subcellular Localization

    Cytoplasm; Nucleus; Cytoplasm, Stress granule; Cytoplasm, P-body

  • Expression


    Tissue_specificity:Ubiquitous. Detected in brain, liver, thymus and muscle

  • Isoforms & Post-Translational Modification

    Q15717 has 2 isomers: Q15717-1: 36092 Da (predicted); Q15717-2: 38996 Da (predicted).
    Phosphorylated by MAPKAPK2 (PubMed:14517288). Phosphorylated by PRKCD (PubMed:18285462);Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge

  • Subunit

    Monomer and homodimer (in vitro) (PubMed:17632515, PubMed:20219472). Interacts with ANP32A (PubMed:11729309). Interacts with ZNF385A; the interaction is indirect and mRNA-dependent and may regulate p53/TP53 expression (By similarity). Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1 (PubMed:19029303). Interacts with AGO1 and AGO2 (PubMed:17932509). Interacts with IGF2BP1; the interaction is enhanced by SEPIN14P20 peptide RBPR (PubMed:29476152, PubMed:32245947). Interacts with IGF2BP2 and IGF2BP3 (PubMed:23640942, PubMed:29476152). Interacts with HNRNPL (PubMed:18161049). Interacts with DHX36; this interaction occurs in a RNA-dependent manner (PubMed:14731398). Interacts with ILF3; this interaction occurs in a RNA-dependent manner (PubMed:14731398). Interacts with PLEKHN1 (PubMed:18191643, PubMed:27616329). Interacts with SHFL; the interaction increases in presence of RNA (PubMed:27974568). Interacts with YBX1; interaction recruits ELAVL1 on C5-methylcytosine (m5C)-containing mRNAs, thereby promoting mRNA stability (PubMed:31358969). Interacts with FXR1 (PubMed:30067974)

  • SwissProt ID

    Q15717

  • Gene ID
    1994 [NCBI]
  • Synonyms

    HUR; Hua; MelG; ELAV1

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Finan JM, et al. Targeting the RNA-Binding Protein HuR in Cancer. Cancer Res. 2023 Nov 1;83(21):3507-3516. [Content Brief]

[2]. Majumder M, et al. HuR as a molecular target for cancer therapeutics and immune-related disorders. Adv Drug Deliv Rev. 2022 Sep;188:114442. [Content Brief]

HuR Antibody (YA1933) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using HuR Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using HuR Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling HuR Antibody (YA1933) (HY-P82188, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • HuR Antibody (YA1933) Product Image
    1/16
  • Experimental Validation Results for HuR Antibody (YA1933)
  • Experimental Validation Results for HuR Antibody (YA1933)
  • Experimental Validation Results for HuR Antibody (YA1933)
  • HuR Antibody (YA1933) Product Image
  • Experimental Validation Results for HuR Antibody (YA1933)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using HuR Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HuR Antibody (YA1933)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using HuR Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HuR Antibody (YA1933)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling HuR Antibody (YA1933) (HY-P82188, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for HuR Antibody (YA1933)
  • Experimental Validation Results for HuR Antibody (YA1933)
  • Experimental Validation Results for HuR Antibody (YA1933)
  • HuR Antibody (YA1933) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using HuR Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HuR Antibody (YA1933) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using HuR Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HuR Antibody (YA1933) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling HuR Antibody (YA1933) (HY-P82188, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • HuR Antibody (YA1933) Product Image IHC-P
  • HuR Antibody (YA1933) Product Image IHC-P
  • HuR Antibody (YA1933) Product Image FC