Copy Link Print Information

FSP27/CIDEC Antibody (YA911)

(Synonyms: Cell Death Activator; Cell death activator CIDE-3; Cell death inducing DFFA like effector protein C; CIDE 3; CIDE C; CIDEC_HUMAN; FLJ20871; FPLD5; FSP27; CIDE-3)
Customer Review

Based on 1 Customer Validation

FSP27/CIDEC Antibody (YA911) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to FSP27/CIDEC.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FSP27/CIDEC Antibody (YA911)
  • FSP27/CIDEC Antibody (YA911) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

FSP27/CIDEC Antibody (YA911) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to FSP27/CIDEC.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 34 kD Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity Database

Entrez Gene: 63924 Human

SwissProt: Q96AQ7 Human

Immunogen

Purified recombinant fragment of human CIDEC expressed in E. Coli.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FSP27/CIDEC Antibody (YA911)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 1), A549 (lane 2), Mouse heart (lane 3), and Mouse liver (lane 4) using FSP27/CIDEC antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    FSP27/CIDEC is a Lipid transferase specifically expressed in white adipose tissue, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion. Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage. Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by undergoing liquid-liquid phase separation (LLPS) and promoting directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1. May also act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment[1][2][3][4][5][6].

  • Subcellular Localization

    Lipid droplet; Endoplasmic reticulum; Nucleus

  • Expression


    Tissue_specificity:It is mainly expressed in adipose tissue, small intestine, heart, colon and stomach, with lower expression levels in brain, kidney and liver.

  • Isoforms & Post-Translational Modification

    Q96AQ7 has 4 isomers: Q96AQ7-1: 26754 Da (predicted); Q96AQ7-2: 18439 Da (predicted); Q96AQ7-3: 14013 Da (predicted); Q96AQ7-4: 27923 Da (predicted).
    Ubiquitinated and targeted to proteasomal degradation, resulting in a short half-life (about 15 minutes in 3T3-L1 cells). Protein stability depends on triaclyglycerol synthesis, fatty acid availability and lipid droplet formation

  • Subunit

    Homodimer (By similarity). Homooligomer; undergoes liquid-liquid phase separation (LLPS) via its N-terminus, facilitating lipid droplet fusion, occurs at the lipid droplet contact sites (By similarity). Interacts with CIDEA (PubMed:19843876). Interacts with PLIN1 (PubMed:23399566). Interacts with NFAT5; this interaction is direct and retains NFAT5 in the cytoplasm (By similarity). Interacts with CEBPB (By similarity). Interacts with isoform CLSTN3beta of CLSTN3; inhibiting the lipid transferase activity of CIDEC (By similarity)

  • SwissProt ID

    Q96AQ7

  • Gene ID
    63924 [NCBI]
  • Synonyms

    Cell Death Activator; Cell death activator CIDE-3; Cell death inducing DFFA like effector protein C; CIDE 3; CIDE C; CIDEC_HUMAN; FLJ20871; FPLD5; FSP27; CIDE-3

  • Research Field

    Cell Biology

[1]. Keller P, et al. Fat-specific protein 27 regulates storage of triacylglycerol. J Biol Chem. 2008 May 23;283(21):14355-65. [Content Brief]

[2]. Liu K, et al. Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA. Am J Physiol Endocrinol Metab. 2009 Dec;297(6):E1395-413. [Content Brief]

[3]. Rubio-Cabezas O, et al. Partial lipodystrophy and insulin resistant diabetes in a patient with a homozygous nonsense mutation in CIDEC. EMBO Mol Med. 2009 Aug;1(5):280-7. [Content Brief]

[4]. Grahn TH, et al. FSP27 and PLIN1 interaction promotes the formation of large lipid droplets in human adipocytes. Biochem Biophys Res Commun. 2013 Mar 8;432(2):296-301. [Content Brief]

[5]. Wang J, et al. Polybasic RKKR motif in the linker region of lipid droplet (LD)-associated protein CIDEC inhibits LD fusion activity by interacting with acidic phospholipids. J Biol Chem. 2018 Dec 14;293(50):19330-19343. [Content Brief]

[6]. Liang L, et al. Molecular cloning and characterization of CIDE-3, a novel member of the cell-death-inducing DNA-fragmentation-factor (DFF45)-like effector family. Biochem J. 2003 Feb 15;370(Pt 1):195-203. [Content Brief]

View More

FSP27/CIDEC Antibody (YA911) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 1), A549 (lane 2), Mouse heart (lane 3), and Mouse liver (lane 4) using FSP27/CIDEC antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • FSP27/CIDEC Antibody (YA911) Product Image
    1/16
  • Experimental Validation Results for FSP27/CIDEC Antibody (YA911)
  • FSP27/CIDEC Antibody (YA911) Product Image
  • Experimental Validation Results for FSP27/CIDEC Antibody (YA911)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 1), A549 (lane 2), Mouse heart (lane 3), and Mouse liver (lane 4) using FSP27/CIDEC antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for FSP27/CIDEC Antibody (YA911)
  • FSP27/CIDEC Antibody (YA911) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 1), A549 (lane 2), Mouse heart (lane 3), and Mouse liver (lane 4) using FSP27/CIDEC antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • FSP27/CIDEC Antibody (YA911) Product Image WB