Cadherin Antibody (YA5528)
(Synonyms: Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; Cadherin-2; CDw325; Neural cadherin; N-cadherin1 Publication; CD325; Cadherin-3; Placental cadherin; P-cadherin; Cadherin-4; Retinal cadherin; R-CAD; R-cadherin)Cadherin Antibody (YA5528) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Cadherin.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, WB, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1000 | 1:500-2000 | 1:100-500 | 1:1000-5000 |
Product Details
Cadherin Antibody (YA5528) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Cadherin.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Synthesized peptide derived from human cadherin-1 AA range:800-882 according to Cadherin-1
Protein G
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Cadherins are a family of calcium-dependent cell adhesion molecules and transmembrane glycoproteins that play a crucial role in maintaining tissue architecture and cellular communication. Key members of the cadherin family include E-cadherin (CDH1), N-cadherin (CDH2), and P-cadherin (CDH3), etc. Cadherins mediate cell-cell adhesion through homophilic interactions, forming adherens junctions that connect to the actin cytoskeleton via catenins, thus influencing cellular signaling pathways related to proliferation, differentiation, and migration[1].
cadherins are involved in various signaling pathways, such as the Wnt and Notch pathways, which are critical for development and tissue homeostasis. Cadherins often bind to β-catenin (CTNNB1) through the catenin-binding domain. The catenin-binding domain of cadherin is crucial in cadherin function and it plays an important role in maintaining epithelial integrity. Dysregulation of cadherin expression is associated with several diseases, particularly cancer, such as pancreatic cancer, melanoma, hepatocellular carcinoma, glioblastoma, breast and gastric cancers. The epigenetic silencing of cadherins through mechanisms like DNA methylation further exacerbates this issue in tumor progression. Understanding cadherins' roles in cellular dynamics provides insights into potential therapeutic targets for cancer and other diseases characterized by altered cell adhesion[1]. -
Subcellular Localization
P55285: Cell membrane; Single-pass type I membrane protein
P22223: Cell membrane; Single-pass type I membrane protein
P12830: Cell junction, adherens junction; Cell membrane; Single-pass type I membrane protein; Endosome; Golgi apparatus, trans-Golgi network; Cytoplasm; Cell junction, desmosome -
Expression
Tissue_specificity: P55285: Highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas
P22223: Expressed in some normal epithelial tissues and in some carcinoma cell lines
P12830: Expressed in granuloma macrophages (at protein level) (PubMed:27760340). Expressed in the skin (at protein level) (PubMed:22294297). Expressed in the liver (PubMed:3263290)
P55283: Expressed mainly in brain but also found in other tissues
Induction: P12830: Induced in the hours following cyclic mechanical strain in keratinocytes (PubMed:31835537). Expression is repressed by MACROD1 (PubMed:17893710) -
Subunit
P22223: Interacts with CDCP1 and CTNNB1
P12830: Homodimer; disulfide-linked (PubMed:11856755). Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1, beta-catenin/CTNNB1 or gamma-catenin/JUP, and potentially alpha-catenin/CTNNA1. -
Synonyms
Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; Cadherin-2; CDw325; Neural cadherin; N-cadherin1 Publication; CD325; Cadherin-3; Placental cadherin; P-cadherin; Cadherin-4; Retinal cadherin; R-CAD; R-cadherin
Documentation
References
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