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CD63 Antibody (YA530)

(Synonyms: CD63 antigen; CD63)
3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

CD63 Antibody (YA530) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD63.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Mouse

  • Formulation:

    1.Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
    2.Supplied in PBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD63 Antibody (YA530)
  • Experimental Validation Results for CD63 Antibody (YA530)
  • CD63 Antibody (YA530) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) CD63 Antibody (YA530)

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3 Publications Citing Use of MCE CD63 Antibody (YA530) (1)
WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

CD63 Antibody (YA530) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD63.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 40-70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 26 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Mouse CD63.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102625

Product Properties

  • Appearance

    Solution

  • Formulation

    1.Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
    2.Supplied in PBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
    Please refer to the lot-specific COA for specific buffer information.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD63 Antibody (YA530)
    Western blot analysis of extracts from RAW264.7(lane 2(20μg) and RAW264.7(lane 3(40μg) using CD63 Antibody (HY-P80604) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    CD63, as a cell surface receptor for TIMP1, participates in the activation of the cell signaling cascade. CD63 participates in the activation of ITGB1 and integrin signals, thereby activating AKT, FAK/PTK2 and MAP kinases. CD63 promotes cell survival, actin cytoskeleton remodeling, cell adhesion, spreading and migration by activating AKT and FAK/PTK2. CD63 participates in the VEGFA signaling pathway by regulating the endocytosis of KDR/VEGFR2. CD63 participates in intracellular vesicle transport and is essential for the normal transport of the PMEL luminal domain, which is crucial for the development and maturation of melanocytes. CD63 participates in the adhesion of leukocytes to endothelial cells by regulating the transport of SELP. CD63 may play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but does not play a role in degranulation in response to other stimuli[1].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Lysosome membrane; Multi-pass membrane protein; Late endosome membrane; Multi-pass membrane protein; Endosome, multivesicular body; Melanosome; Secreted, extracellular exosome; Cell surface

  • Expression


    Tissue_specificity:Ubiquitous. Strongly expressed in kidney. Detected in spleen, bone marrow, peripheral blood mononuclear cells and macrophages

  • Subunit

    Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1. Interacts with CD9. Identified in a complex with CD9 and ITGB3. Interacts with PMEL.

  • SwissProt ID

    P41731

  • Gene ID
    967 [NCBI]
  • Synonyms

    CD63 antigen; CD63

  • Research Field

    Cardiovascular

[1]. Palmulli R, et al. CD63 sorts cholesterol into endosomes for storage and distribution via exosomes. Nat Cell Biol. 2024 Jul;26(7):1093-1109. [Content Brief]

CD63 Antibody (YA530) Related Classifications

100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    CD63 Antibody (YA530) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20μg) and RAW264.7(lane 3(40μg) using CD63 Antibody (HY-P80604) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    CD63 Antibody (YA530) purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Jan 31;23(1):142. [Abstract]

    Customer Validation
     Western blot analysis detecting the expression of exosome marker proteins CD63 and TSG101.

  • CD63 Antibody (YA530) Product Image
    1/16
  • Experimental Validation Results for CD63 Antibody (YA530)
  • Experimental Validation Results for CD63 Antibody (YA530)
  • CD63 Antibody (YA530) Product Image
  • Experimental Validation Results for CD63 Antibody (YA530)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20μg) and RAW264.7(lane 3(40μg) using CD63 Antibody (HY-P80604) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD63 Antibody (YA530)
  • CD63 Antibody (YA530) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20μg) and RAW264.7(lane 3(40μg) using CD63 Antibody (HY-P80604) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD63 Antibody (YA530) Product Image WB