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ATF6 Antibody (YA831)

(Synonyms: Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha, ATF6)
2 Cited Publications
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Based on 2 publication(s) in Google Scholar

ATF6 Antibody (YA831) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATF6.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATF6 Antibody (YA831)
  • Experimental Validation Results for ATF6 Antibody (YA831)
  • ATF6 Antibody (YA831) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:5000 1:50-1:100 1:50-1:100

Product Details

Description

ATF6 Antibody (YA831) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATF6.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 75 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human ATF6.AA range:1-670.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102467

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATF6 Antibody (YA831)
    Western blot analysis of extracts from Hela(lane 2(20μg) , jurkat(lane 3(20μg) and RAW264.7(lane 4(20ug) using ATF6 (HY-P80379 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATF6 Antibody (YA831)
    Flow cytometric analysis of 1X106 HeLa cells labeling ATF6 Antibody (HY-P80379, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    ATF6 (Activating transcription factor 6) is an endoplasmic reticulum (ER) -localised protein and member of the leucine zipper family of transcription factors. ATF6 plays an important role in transducing signals linked to the ER stress. In the absence of ER stress, GRP78 binds to the ER luminal domain of ATF6 and anchors ATF6 in the ER. Upon ER stress, GRP78 dissociates from, and thus, releases ATF6, allowing it to relocate to the next destination in the secretory pathway, the Golgi. In the Golgi, ATF6 is cleaved by S1P and S2P proteases by regulated intramembrane proteolysis. This cleavage liberates the N-terminal 50 kD cytosolic portion of ATF6 (activated form), which has a nuclear localization sequence, facilitating its movement to the nucleus, where it acts as a transcription factor to induce genes that encode stress-reducing chaperones and foldases that slow the pace of protein translation.

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass type II membrane protein; Golgi apparatus membrane; Single-pass type II membrane protein; Nucleus

  • Expression


    Tissue_specificity:general expression

  • Subunit

    Interacts with XBP1 isoform 2; the interaction occurs in a ER stress-dependent manner (PubMed:17765680). Interacts with LACC1 (PubMed:31875558)

  • SwissProt ID

    P18850

  • Gene ID
    22926 [NCBI]
  • Synonyms

    Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha, ATF6

  • Research Field

    Epigenetics and Nuclear Signaling

ATF6 Antibody (YA831) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , jurkat(lane 3(20μg) and RAW264.7(lane 4(20ug) using ATF6 (HY-P80379 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling ATF6 Antibody (HY-P80379, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ATF6 Antibody (YA831) Product Image
    1/16
  • Experimental Validation Results for ATF6 Antibody (YA831)
  • Experimental Validation Results for ATF6 Antibody (YA831)
  • ATF6 Antibody (YA831) Product Image
  • Experimental Validation Results for ATF6 Antibody (YA831)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , jurkat(lane 3(20μg) and RAW264.7(lane 4(20ug) using ATF6 (HY-P80379 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATF6 Antibody (YA831)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling ATF6 Antibody (HY-P80379, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for ATF6 Antibody (YA831)
  • Experimental Validation Results for ATF6 Antibody (YA831)
  • ATF6 Antibody (YA831) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg) , jurkat(lane 3(20μg) and RAW264.7(lane 4(20ug) using ATF6 (HY-P80379 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ATF6 Antibody (YA831) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling ATF6 Antibody (HY-P80379, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ATF6 Antibody (YA831) Product Image WB
  • ATF6 Antibody (YA831) Product Image FC