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SF3B1 Antibody (YA2256)

(Synonyms: SF3B1; SAP155; Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155)
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SF3B1 Antibody (YA2256) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SF3B1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • SF3B1 Antibody (YA2256) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200 1:50-1:100

Product Details

Description

SF3B1 Antibody (YA2256) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SF3B1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 155 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 146 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human SF3B1 aa1-53/1304.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    SF3B1 is the largest subunit of the spliceosome factor 3b (SF3B) complex and functions by serving as a core component of the 17S U2 SnRNP complex of the spliceosome. The spliceosome removes introns from precursor mRNA (premRNA) and generates mature, spliced mRNAs. SF3B1 mutations induce common and tumor-specific splicing aberrations[1].

  • Subcellular Localization

    Nucleus; Nucleus speckle

  • Isoforms & Post-Translational Modification

    O75533 has 2 isomers: O75533-1: 145830 Da (predicted); O75533-2: 16020 Da (predicted).
    Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis;Citrullinated by PADI4

  • Subunit

    Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins (PubMed:30567737, PubMed:32494006, PubMed:34822310, PubMed:36797247). Part of the SF3B subcomplex of the 17S U2 SnRNP complex (PubMed:12234937, PubMed:12738865, PubMed:27720643, PubMed:28541300). SF3B associates with the splicing subcomplex SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP) (PubMed:12234937). Within the SF3B complex, interacts directly (via HEAT domain) with SF3B3, SF3B5, SF3B6 and (via HEAT domain) with PHF5A (PubMed:16432215, PubMed:21062891, PubMed:27720643, Ref.39). The SF3B subcomplex interacts with U2AF2 (PubMed:27720643). Identified in the spliceosome C complex (PubMed:11991638). Component of the minor (U12-type spliceosome) spliceosome (PubMed:15146077, PubMed:33509932). Within the minor spliceosome complex, interacts with SCNM1 and CRIPT (PubMed:33509932). Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21 (PubMed:16603771). Phosphorylated form interacts with PPP1R8 (PubMed:12105215). Interacts with PQBP1 (PubMed:23512658). Interacts with RBM17 (PubMed:17589525). Interacts with RBM39 (PubMed:24795046). Interacts with SETX (PubMed:21700224). Interacts with RBM15 (PubMed:26575292). Interacts with USH1G (PubMed:34023904). Interacts with SDE2 (PubMed:34365507). Interacts with U2AF1 (PubMed:34365507). Interacts with CACTIN (PubMed:34365507). Interacts with ZRSR1 (By similarity). Interacts with CYREN (PubMed:37813856)

  • SwissProt ID

    O75533

  • Gene ID
    23451 [NCBI]
  • Synonyms

    SF3B1; SAP155; Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Zhixia Zhou, et al. The biological function and clinical significance of SF3B1 mutations in cancer. Biomark Res. 2020 Sep 3:8:38. [Content Brief]

SF3B1 Antibody (YA2256) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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