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GST-Tag Antibody (YA863)

(Synonyms: Glutathione S Transferase antibody; Glutathione S-transferase class-mu 26 kDa isozyme antibody; GST antibody; Sj26 antigen antibody; SjGST antibody)
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Based on 1 Customer Validation

GST-Tag Antibody (YA863) is an unconjugated, mouse-derived, anti-GST-Tag IgG1 monoclonal antibody.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GST-Tag Antibody (YA863)
  • GST-Tag Antibody (YA863) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:50000 1:50-1:200 Use at an assay dependent concentration.

Product Details

Description

GST-Tag Antibody (YA863) is an unconjugated, mouse-derived, anti-GST-Tag IgG1 monoclonal antibody.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Immunogen

GST tag recombinant protein.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103028

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GST-Tag Antibody (YA863)
    Western blot analysis of CALR (HY-P72112, lane 2(5ng) and CALR (HY-P72112, lane 3(10ng)using GST-Tag (HY-P80148A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80148A, 1/1000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    GST-Tag is a The GST tag is an affinity tag composed of glutathione S-transferase and is widely used in the purification, soluble expression, and protein-protein interaction studies of recombinant proteins.

  • Expression


    Positive sample: 293T transfected with GST cell lysate, 293T transfected with GST-tagged Histone H3.1 cell lysate, HeLa transfected with GST-tagged Histone H3.1 cells.

  • Synonyms

    Glutathione S Transferase antibody; Glutathione S-transferase class-mu 26 kDa isozyme antibody; GST antibody; Sj26 antigen antibody; SjGST antibody

  • Research Field

    Tags & Cell Markers

GST-Tag Antibody (YA863) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of CALR (HY-P72112, lane 2(5ng) and CALR (HY-P72112, lane 3(10ng)using GST-Tag (HY-P80148A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80148A, 1/1000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • GST-Tag Antibody (YA863) Product Image
    1/16
  • Experimental Validation Results for GST-Tag Antibody (YA863)
  • GST-Tag Antibody (YA863) Product Image
  • Experimental Validation Results for GST-Tag Antibody (YA863)
    1/1
    MedchemExpress Validation
     Western blot analysis of CALR (HY-P72112, lane 2(5ng) and CALR (HY-P72112, lane 3(10ng)using GST-Tag (HY-P80148A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80148A, 1/1000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GST-Tag Antibody (YA863)
  • GST-Tag Antibody (YA863) Product Image
    MedchemExpress Validation

    Western blot analysis of CALR (HY-P72112, lane 2(5ng) and CALR (HY-P72112, lane 3(10ng)using GST-Tag (HY-P80148A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80148A, 1/1000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • GST-Tag Antibody (YA863) Product Image WB