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Ephrin Receptor B4 Antibody (YA2892)

(Synonyms: EPHB4; HTK; MYK1; TYRO11; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; Tyrosine-protein kinase TYRO11)
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Based on 1 Customer Validation

Ephrin Receptor B4 Antibody (YA2892) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ephrin Receptor B4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Ephrin Receptor B4 Antibody (YA2892) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Ephrin Receptor B4 Antibody (YA2892) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ephrin Receptor B4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 135 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 108 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human Eph receptor B4/HTK aa17-529.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105262

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
    Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), HUVEC (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), A431 (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using Eph receptor B4/HTK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
    Western blot analysis of extracts from Hela (lane 2(20μg) , A549 (lane 3(20μg), and C6 (lane 4(20μg) using Ephrin Receptor B4 (HY-P83147) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue using PRK2 Antibody (YA2890). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83145, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue using PRK2 Antibody (YA2890). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83145, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Ephrin Receptor B4 receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is highly expressed in the placenta, but has also been detected in the kidneys, liver, lungs, pancreas, skeletal muscle, and heart. It is expressed in primitive hematopoietic cells and myeloid hematopoietic cells, but not in lymphoid hematopoietic cells. It has also been observed in cell lines derived from the liver, mammary gland, colon, lung, melanocytes, and cervix.

  • Isoforms & Post-Translational Modification

    P54760 has 4 isomers: P54760-1: 108270 Da (predicted); P54760-2: 55975 Da (predicted); P54760-3: 33342 Da (predicted); P54760-4: 45195 Da (predicted).
    Phosphorylated; autophosphorylation is stimulated by EFNB2

  • Subunit

    Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer.

  • SwissProt ID

    P54760

  • Gene ID
    2050 [NCBI]
  • Synonyms

    EPHB4; HTK; MYK1; TYRO11; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; Tyrosine-protein kinase TYRO11

  • Research Field

    Cardiovascular

Ephrin Receptor B4 Antibody (YA2892) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), HUVEC (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), A431 (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using Eph receptor B4/HTK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , A549 (lane 3(20μg), and C6 (lane 4(20μg) using Ephrin Receptor B4 (HY-P83147) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue using PRK2 Antibody (YA2890). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83145, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue using PRK2 Antibody (YA2890). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83145, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Ephrin Receptor B4 Antibody (YA2892) Product Image
    1/16
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Ephrin Receptor B4 Antibody (YA2892) Product Image
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), HUVEC (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), A431 (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using Eph receptor B4/HTK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , A549 (lane 3(20μg), and C6 (lane 4(20μg) using Ephrin Receptor B4 (HY-P83147) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue using PRK2 Antibody (YA2890). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83145, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue using PRK2 Antibody (YA2890). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83145, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Experimental Validation Results for Ephrin Receptor B4 Antibody (YA2892)
  • Ephrin Receptor B4 Antibody (YA2892) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), HUVEC (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), A431 (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using Eph receptor B4/HTK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Ephrin Receptor B4 Antibody (YA2892) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) , A549 (lane 3(20μg), and C6 (lane 4(20μg) using Ephrin Receptor B4 (HY-P83147) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Ephrin Receptor B4 Antibody (YA2892) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue using PRK2 Antibody (YA2890). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83145, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Ephrin Receptor B4 Antibody (YA2892) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue using PRK2 Antibody (YA2890). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83145, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Ephrin Receptor B4 Antibody (YA2892) Product Image WB
  • Ephrin Receptor B4 Antibody (YA2892) Product Image WB
  • Ephrin Receptor B4 Antibody (YA2892) Product Image IHC-P
  • Ephrin Receptor B4 Antibody (YA2892) Product Image IHC-P