Ephrin Receptor B4 Antibody (YA2892)

Cat. No.: HY-P83147: Data Sheet COA
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Ephrin Receptor B4 Antibody (YA2892) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ephrin Receptor B4.

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50 μL	해외재고보유	Estimated Time of Arrival: December 31
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Ephrin Receptor B4 Antibody (YA2892) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Ephrin Receptor B4 Antibody (YA2892) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ephrin Receptor B4.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 108 kDa;

Observed band size: 135 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P54760

Gene ID

2050 [NCBI]

Immunogen

Recombinant protein of human Eph receptor B4/HTK aa17-529.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

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ALL WB IHC-P

Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), HUVEC (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), A431 (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using Eph receptor B4/HTK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from Hela (lane 2(20μg) , A549 (lane 3(20μg), and C6 (lane 4(20μg) using Ephrin Receptor B4 (HY-P83147) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue using PRK2 Antibody (YA2890). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83145, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue using PRK2 Antibody (YA2890). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83145, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells

Subcellular Localization：Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:It is highly expressed in the placenta, but has also been detected in the kidneys, liver, lungs, pancreas, skeletal muscle, and heart. It is expressed in primitive hematopoietic cells and myeloid hematopoietic cells, but not in lymphoid hematopoietic cells. It has also been observed in cell lines derived from the liver, mammary gland, colon, lung, melanocytes, and cervix.

Isoforms & Post-Translational Modification：P54760 has 4 isomers: P54760-1: 108270 Da (predicted); P54760-2: 55975 Da (predicted); P54760-3: 33342 Da (predicted); P54760-4: 45195 Da (predicted).
Phosphorylated; autophosphorylation is stimulated by EFNB2

Subunit：Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer.

RRID

AB_3105262

Database

Entrez Gene: 2050 Human ; 13846 Mouse ;

SwissProt: P54760 Human ; P54761 Mouse ;

OMIM: 617300 Human

Research Field

Cardiovascular

Synonyms

EPHB4; HTK; MYK1; TYRO11; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; Tyrosine-protein kinase TYRO11

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Ephrin Receptor B4 Antibody (YA2892) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Ephrin Receptor B4 Antibody (YA2892)

Ephrin Receptor B4 Antibody (YA2892) Featured Recommendations:

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