FBXO22 Antibody (YA8026)(PBS only)

Cat. No.: HY-P88342A: Data Sheet User Guide for Antibodies
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FBXO22 Antibody (YA8026) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FBXO22.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

FBXO22 Antibody (YA8026) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FBXO22.

Host

Mouse

Molecular Weight

Predicted band size: 44.3 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8NEZ5

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-220 of human FBXO22 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex that is implicated in the control of various cellular processes such as cell cycle control, transcriptional regulation, DNA damage repair, and apoptosis. Promotes the proteasome-dependent degradation of key sarcomeric proteins, such as alpha-actinin (ACTN2) and filamin-C (FLNC), essential for maintenance of normal contractile function. Acts as a key regulator of histone methylation marks namely H3K9 and H3K36 methylation through the regulation of histone demethylase KDM4A protein levels (PubMed:21768309). In complex with KDM4A, also regulates the abundance of TP53 by targeting methylated TP53 for degradation at the late senescent stage (PubMed:26868148). Under oxidative stress, promotes the ubiquitination and degradation of BACH1. Mechanistically, reactive oxygen species (ROS) covalently modify cysteine residues on the bZIP domain of BACH1, leading to its release from chromatin and making it accessible to FBXO22 (PubMed:39504958). Upon amino acid depletion, mediates 'Lys-27'-linked ubiquitination of MTOR and thereby inhibits substrate recruitment to mTORC1 (PubMed:37979583). Also inhibits SARS-CoV-2 replication by inducing NSP5 degradation (PubMed:39223933)

Subcellular Localization：Cytoplasm,Nucleus,Cytoplasm, myofibril, sarcomere, Z line

Expression：
Tissue_Specificity: Predominantly expressed in liver, also enriched in cardiac muscle

Isoforms & Post-Translational Modification：Q8NEZ5 has three isomers: Q8NEZ5-1: 44508 Da (predicted); Q8NEZ5-2: 4922 Da (predicted); Q8NEZ5-3: 30589 Da (predicted).
Phosphorylated by EIF2AK4 at Thr-127 causes cytoplasmic retention of FBXO22

Subunit：Directly interacts with SKP1 and CUL1 (PubMed:21768309, PubMed:22972877)

Synonyms

FBX22; FISTC1

Documentation

User Guide for Antibodies (1077 KB)