HAS2 Antibody (YA4401)

Cat. No.: HY-P84704: Ficha de datos COA
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HAS2 Antibody (YA4401) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HAS2.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn
Referencias

Descripciòn

HAS2 Antibody (YA4401) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HAS2.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 64 kDa;

Observed band size: 64 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

Q92819

Gene ID

3037 [NCBI]

Immunogen

Purified recombinant fragment of human HAS2 (AA: 67-170) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from HEK293 (lane 2(20μg), Hela (lane 3(20μg), U251 (lane 4(20μg) and U87 (lane 5(20μg) using HAS2 Antibody (HY-P84704) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from PC-12(lane 1(40μg) )
Immunofluorescence analysis of Hela cells labeling HAS2 antibody (HY-P84704) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

Function：Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer (Probable) (PubMed:20507985, PubMed:21228273, PubMed:23303191, PubMed:32993960). Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation (PubMed:20507985, PubMed:21228273, PubMed:8798477). This is one of three isoenzymes responsible for cellular hyaluronan synthesis and it is particularly responsible for the synthesis of high molecular mass hyaluronan (By similarity)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Endoplasmic reticulum membrane; Multi-pass membrane protein; Vesicle; Golgi apparatus membrane; Multi-pass membrane protein; Lysosome

Expression：
Tissue_specificity:Expressed in fibroblasts

Induction:During cell cycle progression is induced at the G1-S and G2-M transitions. Up-regulated in a panel of cancer cell lines

Subunit：Homodimer; dimerization promotes enzymatic activity (PubMed:20507985, PubMed:25795779). Forms heterodimer with HAS3 (PubMed:20507985, PubMed:25795779). Forms heterodimer with HAS1 (PubMed:25795779)

RRID

AB_3718967

Synonyms

Hyaluronan synthase 2, Hyaluronate synthase 2, Hyaluronic acid synthase 2, HA synthase 2, HAS2

Documentación

Select Batch:

Ficha de datos (258 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/du-145-cell.html [Content Brief]