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HAS2 Antibody (YA4401)

(Synonyms: Hyaluronan synthase 2, Hyaluronate synthase 2, Hyaluronic acid synthase 2, HA synthase 2, HAS2)
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Based on 1 Customer Validation

HAS2 Antibody (YA4401) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HAS2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for HAS2 Antibody (YA4401)
  • Experimental Validation Results for HAS2 Antibody (YA4401)
  • Experimental Validation Results for HAS2 Antibody (YA4401)
  • HAS2 Antibody (YA4401) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:100-1:500 1:10000

Product Details

Description

HAS2 Antibody (YA4401) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HAS2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 64 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 64 kDa
Immunogen

Purified recombinant fragment of human HAS2 (AA: 67-170) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718967

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HAS2 Antibody (YA4401)
    Western blot analysis of extracts from HEK293 (lane 2(20μg), Hela (lane 3(20μg), U251 (lane 4(20μg) and U87 (lane 5(20μg) using HAS2 Antibody (HY-P84704) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HAS2 Antibody (YA4401)
    Western blot analysis of extracts from PC-12(lane 1(40μg) )
  • Experimental Validation Results for HAS2 Antibody (YA4401)
    Immunofluorescence analysis of Hela cells labeling HAS2 antibody (HY-P84704) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

  • Function

    HAS2 catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer (Probable). Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis and it is particularly responsible for the synthesis of high molecular mass hyaluronan[1][2][3][4][5].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Endoplasmic reticulum membrane; Multi-pass membrane protein; Vesicle; Golgi apparatus membrane; Multi-pass membrane protein; Lysosome

  • Expression


    Tissue_specificity:Expressed in fibroblasts

    Induction:During cell cycle progression is induced at the G1-S and G2-M transitions. Up-regulated in a panel of cancer cell lines

  • Subunit

    Homodimer; dimerization promotes enzymatic activity (PubMed:20507985, PubMed:25795779). Forms heterodimer with HAS3 (PubMed:20507985, PubMed:25795779). Forms heterodimer with HAS1 (PubMed:25795779)

  • SwissProt ID

    Q92819

  • Gene ID
    3037 [NCBI]
  • Synonyms

    Hyaluronan synthase 2, Hyaluronate synthase 2, Hyaluronic acid synthase 2, HA synthase 2, HAS2

HAS2 Antibody (YA4401) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), Hela (lane 3(20μg), U251 (lane 4(20μg) and U87 (lane 5(20μg) using HAS2 Antibody (HY-P84704) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from PC-12(lane 1(40μg) )

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling HAS2 antibody (HY-P84704) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • HAS2 Antibody (YA4401) Product Image
    1/16
  • Experimental Validation Results for HAS2 Antibody (YA4401)
  • Experimental Validation Results for HAS2 Antibody (YA4401)
  • Experimental Validation Results for HAS2 Antibody (YA4401)
  • HAS2 Antibody (YA4401) Product Image
  • Experimental Validation Results for HAS2 Antibody (YA4401)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), Hela (lane 3(20μg), U251 (lane 4(20μg) and U87 (lane 5(20μg) using HAS2 Antibody (HY-P84704) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HAS2 Antibody (YA4401)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from PC-12(lane 1(40μg) )
  • Experimental Validation Results for HAS2 Antibody (YA4401)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling HAS2 antibody (HY-P84704) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for HAS2 Antibody (YA4401)
  • Experimental Validation Results for HAS2 Antibody (YA4401)
  • Experimental Validation Results for HAS2 Antibody (YA4401)
  • HAS2 Antibody (YA4401) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2(20μg), Hela (lane 3(20μg), U251 (lane 4(20μg) and U87 (lane 5(20μg) using HAS2 Antibody (HY-P84704) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HAS2 Antibody (YA4401) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from PC-12(lane 1(40μg) )

  • HAS2 Antibody (YA4401) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling HAS2 antibody (HY-P84704) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • HAS2 Antibody (YA4401) Product Image WB
  • HAS2 Antibody (YA4401) Product Image WB
  • HAS2 Antibody (YA4401) Product Image ICC