CPSF6 Antibody (YA2554)(PBS only)

Art. -Nr.: HY-P82809A: Data Sheet User Guide for Antibodies
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CPSF6 Antibody (YA2554) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CPSF6.

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung
Verweise

Beschreibung

CPSF6 Antibody (YA2554) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CPSF6.

Host

Rabbit

Molekulargewicht

Predicted band size: 59 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q16630

Gene ID

11052 [NCBI]

Immunogen

A synthetic peptide of human CPSF6

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:500
FC FC: Flow Cytometry	1:20-1:50
IP IP: Immunoprecipitation	1:10-1:100

Reinheit	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), 3T3 (lane 3, 15 μg), C6 (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using CPSF6 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:14690600, PubMed:29276085, PubMed:8626397, PubMed:9659921). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:14690600, PubMed:8626397, PubMed:9659921). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:20695905, PubMed:29276085). CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery (PubMed:15169763, PubMed:21295486, PubMed:29276085). Plays a role in mRNA export (PubMed:19864460); (Microbial infection) Binds HIV-1 capsid-nucleocapsid (HIV-1 CA-NC) complexes and might thereby promote the integration of the virus in the nucleus of dividing cells (in vitro)

Subcellular Localization：Nucleus; Nucleus, nucleoplasm; Nucleus speckle; Cytoplasm

Isoforms & Post-Translational Modification：Q16630 has 3 isomers: Q16630-1: 59210 Da (predicted); Q16630-2: 63471 Da (predicted); Q16630-3: 52326 Da (predicted).
Phosphorylated (PubMed:29276085). Phosphorylated in the Arg/Ser-rich domain by SRPK1, in vitro (PubMed:29276085);Symmetrically dimethylated on arginine residues in the GAR motif by PRMT5 in a WDR77- and CLNS1A-dependent manner (PubMed:20562214). Asymmetrically dimethylated on arginine residues in the GAR motif by PRMT1 (PubMed:20562214)

Subunit：Component of the cleavage factor Im (CFIm) complex which is a heterotetramer composed of two subunits of NUDT21/CPSF5 and two subunits of CPSF6 or CPSF7 or a heterodimer of CPSF6 and CPSF7 (PubMed:14561889, PubMed:20695905, PubMed:23187700, PubMed:8626397, PubMed:9659921).

Research Field

Epigenetics and Nuclear Signaling

Synonyms

CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7

Dokumentation

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/mkn-45-cell.html [Content Brief]