2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. NDUFS8 Antibody (YA1352)

NDUFS8 Antibody (YA1352)

Art. -Nr.: HY-P81607
COA
SDS
User Guide for Antibodies Technical Support

NDUFS8 Antibody (YA1352) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDUFS8.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Beschreibung

Beschreibung

NDUFS8 Antibody (YA1352) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDUFS8.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molekulargewicht
Predicted band size: 24 kDa;
Observed band size: 24 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human NDUFS8
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Reinheit Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using NDUFS8 Antibody (HY-P81607) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:22499348). Essential for the catalytic activity and assembly of complex I (PubMed:22499348)
Subcellular Localization：Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
Expression：
Tissue_specificity:Expressed in all tissues with the highest level in heart and skeletal muscle and the lowest level in lung
Subunit：Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits (PubMed:12611891). This is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity). Interacts with RAB5IF (PubMed:31536960)
RRID
Database

Entrez Gene: 4728 Human ; 225887 Mouse ; 293652 Rat

SwissProt: O00217 Human ; Q8K3J1 Mouse ;

OMIM: 618222 Human

Research Field

Tags & Cell Markers
Synonyms
TYKY; CI-23k; CI23KD
Dokumentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

NDUFS8 Antibody (YA1352) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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NDUFS8 Antibody (YA1352)
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