Shugoshin 1 Antibody
(Synonyms: SGOL1, SGO1, Shugoshin 1, Serologically defined breast cancer antigen NY-BR-85, Shugoshin-like 1)Shugoshin 1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Shugoshin 1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
Shugoshin 1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Shugoshin 1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 24; 29; 31; 33; 35; 60; 64 kDa
Purified recombinant protein of human Shugoshin 1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Shugoshin 1 plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Involved in centromeric enrichment of AUKRB in prometaphase
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Subcellular Localization
Nucleus; Chromosome, centromere; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, spindle pole; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Nucleus speckle
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Expression
Tissue_Specificity: Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested. -
Isoforms & Post-Translational Modification
Shugoshin 1 has 7 isoforms, Q5FBB7-1: amino acid length is 561, molecular weight is 64190 Da (predicted); Q5FBB7-2: amino acid length is 309, molecular weight is 35344 Da (predicted); Q5FBB7-3: amino acid length is 292, molecular weight is 33501 Da (predicted); Q5FBB7-4: amino acid length is 275, molecular weight is 31276 Da (predicted); Q5FBB7-5: amino acid length is 258, molecular weight is 29433 Da (predicted); Q5FBB7-6: amino acid length is 527, molecular weight is 60122 Da (predicted); Q5FBB7-7: amino acid length is 215, molecular weight is 24646 Da (predicted).Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1
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Subunit
Interacts with PPP2CA (or PPP2CB), PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E, SET, LRRC59, RBM10 (or RBM5), RPL10A, RPL28, RPL7, RPL7A and RPLP1.
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SwissProt ID
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Synonyms
SGOL1, SGO1, Shugoshin 1, Serologically defined breast cancer antigen NY-BR-85, Shugoshin-like 1
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